The micronucleus assay in radiation accidents.
The micronucleus assay in radiation accidents.
复制标题
辐射事故中的微核测定。
DOI：
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复制
发表时间：
2009
期刊：
影响因子：
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通讯作者：
A. Vral
中科院分区：
文献类型：
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作者：
H. Thierens;A. Vral
外周血淋巴细胞中的微核阻断试验是一种标准化和经验证的生物剂量测定技术。微核的自动评分允许大规模应用，如在辐射事故或恶意使用放射源的情况下的人群分类。用于个体剂量评估的微核试验的剂量检测限（95%置信度）限于0.2戈伊，但可通过使用荧光原位杂交（FISH）对微核中的着丝粒进行评分降低至0.1戈伊。在过去，微核试验被应用于许多大规模的核电站工人和医院工作人员的生物监测研究。基线微核频率强烈依赖于年龄和性别。该分析也已用于辐射事故的生物剂量测定。在2003年意外暴露于X射线的工人的剂量评估的多终点生物剂量学研究中，获得了良好的协议之间的剂量估计产生的微核试验，双着丝粒和易位的评分。结合着丝粒信号对微核进行自动评分，从而对受照射人群进行系统的生物剂量测定，这仍然是未来的一项挑战。
The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is a standardised and validated technique for biodosimetry. Automated scoring of micronuclei allows large scale applications as in population triage in case of radiation accidents or malevolent use of radioactive sources. The dose detection limit (95% confidence) of the micronucleus assay for individual dose assessment is restricted to 0.2 Gy but can be decreased to 0.1 Gy by scoring centromeres in micronuclei using fluorescence in situ hybridization (FISH). In the past the micronucleus assay was applied for a number of large scale biomonitoring studies of nuclear power plant workers and hospital workers. Baseline micronucleus frequencies depend strongly on age and gender. The assay was also already used for biodosimetry of radiation accidents. In a multiple endpoint biodosimetry study for dose assessment of a worker exposed accidentally in 2003 to X-rays, a good agreement was obtained between dose estimates resulting from the micronucleus assay, the scoring of dicentrics and translocations. Automated scoring of micronuclei in combination with centromere signals, allowing systematic biodosimetry of exposed populations, remains a challenge for the future.
居住在塞米巴拉金斯克核试验场附近的个体淋巴细胞中不稳定型染色体畸变
DOI：
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发表时间：
2006
期刊：
Journal of Radiation Research 47 Supplement A
影响因子：
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作者：
Khailov;A.;平林今日子;片山博昭;川野徳幸;V.F.Stepanenko;V.F.Stepanenko;H.Sato;H.Y.Goksu;A.Ivannikov;K.Zhumadilov;A.Romanyukha;V.Skvortsov;S.Toyoda;A.Sevan'kaev;K.Tanaka;M.Yamamoto;N.Semioshkina;A.Sakaguchi;E.Tieliewuhan;T.Imanaka;K.Gordeev;K.Gordeev;S.L.Simon;V.F.Stepanenko;K.Tanaka
通讯作者：
K.Tanaka