Comparative genomics approach to the expression of figα, one of the earliest marker genes of oocyte differentiation in medaka (Oryzias latipes)
Comparative genomics approach to the expression of figα, one of the earliest marker genes of oocyte differentiation in medaka (Oryzias latipes)
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DOI：
10.1016/j.gene.2008.07.007
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发表时间：
2008-11-01
期刊：
影响因子：
3.5
通讯作者：
Hori, Hiroshi
中科院分区：
文献类型：
--
作者：
Kanamori, Akira;Toyama, Keiko;Hori, Hiroshi
我们通过检测卵母细胞表达基因的转录调控，分析了medaka性腺性别分化的分子级联。我们首先通过定量RT-PCR和原位杂交证实figa是最早的卵母细胞分化标记基因之一。推测figa靶基因zpc4和zpb的表达紧随figa α。减数分裂特异性基因scp3的表达在时间和空间上与图α相似。为了表征fig α的顺式调控序列，我们比较了脊椎动物fig α基因的基因组组织。c端外显子的数量和序列同源性均存在差异。暗示他们不太重要的角色。在热带非洲爪蟾和许多硬骨鱼中，fig - α位于己糖激酶2和-内收蛋白之间。我们比较了这个基因组区域潜在的顺式调控元件，没有发现高同源性的DNA片段。尽管缺乏序列相似性，但从河豚或河豚紧凑基因组中获得的含有fig α侧翼序列的荧光蛋白转基因，忠实地再现了medaka卵母细胞中fig α的内源性表达，表明了保守的调控机制。(c) 2008 Elsevier B.V.版权所有
We analyzed molecular cascades of sex differentiation in medaka gonads by examining the transcriptional regulation of the oocyte-expressed gene, fig alpha. We first confirmed that figa is one of the earliest marker genes of oocyte differentiation by quantitative RT-PCR and in situ hybridization. Expression of putative figa target genes, zpc4 and zpb, followed that of figa alpha. A meiosis-specific gene, scp3, showed expression temporally and spatially similar to fig alpha. To characterize the cis-regulatory sequences of fig alpha, we compared genomic organizations of vertebrate fig alpha genes. Both number and sequence homology of the C-terminal exons showed divergence. suggesting their less important roles. In the frog, Xenopus tropicalis, and in many teleosts, fig alpha is located between hexokinase 2 and beta-adducin. We compared this genomic region for potential cis-regulatory elements and found no DNA stretches with high homology. In spite of this lack of sequence similarities, fluorescent protein transgenes surrounded with fig alpha flanking sequences from the compact genomes of fugu or Tetroodon faithfully reproduced the endogenous expression of fig alpha in the medaka oocytes, indicating conserved regulatory mechanisms. (c) 2008 Elsevier B.V. All rights reserved.