Significant effect of HIV/HAART on oral microbiota using multivariate analysis
Significant effect of HIV/HAART on oral microbiota using multivariate analysis
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DOI：
10.1038/s41598-019-55703-9
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发表时间：
2019-12-27
影响因子：
4.6
通讯作者：
Fidel, Paul L., Jr.
中科院分区：
文献类型：
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作者：
Griffen, Ann L.;Thompson, Zachary A.;Fidel, Paul L., Jr.
艾滋病毒感染者特别容易感染各种口腔微生物疾病。虽然各种研究设计和检测方法已被用于比较艾滋病毒阴性和艾滋病毒阳性的人，无论有没有高效抗逆转录病毒治疗（HAART）的口腔微生物群，方法各不相同，结果并不一致或结论性。本研究的目的是使用16S rRNA基因序列分析比较HAART下HIV阳性者与HIV阴性者的口腔细菌群落组成。收集了大量的临床数据，并努力在临床变量上平衡各组，以尽量减少混淆。多变量分析用于评估HIV状态的独立贡献。89名HIV阴性参与者和252名HIV阳性参与者接受HAART。HAART下HIV对口腔微生物组的独立影响具有统计学意义，但小于牙龈炎，牙周病，吸烟，龋齿和其他临床变量的影响。总之，一个大样本的艾滋病毒感染者的多变量比较下HAART的艾滋病毒阴性对照组显示了一组复杂的临床特征，影响口腔细菌群落的组成，包括艾滋病毒的存在下HAART。
Persons infected with HIV are particularly vulnerable to a variety of oral microbial diseases. Although various study designs and detection approaches have been used to compare the oral microbiota of HIV-negative and HIV-positive persons, both with and without highly active antiretroviral therapy (HAART), methods have varied, and results have not been consistent or conclusive. The purpose of the present study was to compare the oral bacterial community composition in HIV-positive persons under HAART to an HIV-negative group using 16S rRNA gene sequence analysis. Extensive clinical data was collected, and efforts were made to balance the groups on clinical variables to minimize confounding. Multivariate analysis was used to assess the independent contribution of HIV status. Eighty-nine HIV-negative participants and 252 HIV-positive participants under HAART were sampled. The independent effect of HIV under HAART on the oral microbiome was statistically significant, but smaller than the effect of gingivitis, periodontal disease, smoking, caries, and other clinical variables. In conclusion, a multivariate comparison of a large sample of persons with HIV under HAART to an HIV-negative control group showed a complex set of clinical features that influenced oral bacterial community composition, including the presence of HIV under HAART.