Heterotypy in the N-terminal region of growth/differentiation factor 5 (GDF5) mature protein during teleost evolution.
Heterotypy in the N-terminal region of growth/differentiation factor 5 (GDF5) mature protein during teleost evolution.
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DOI：
10.1093/molbev/msn041
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发表时间：
2008-05
影响因子：
10.7
通讯作者：
Koji Fujimura;Y. Terai;Naoya B. Ishiguro;M. Miya;M. Nishida;N. Okada
中科院分区：
文献类型：
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作者：
Koji Fujimura;Y. Terai;Naoya B. Ishiguro;M. Miya;M. Nishida;N. Okada
异型性现在被认为是通过修饰现有蛋白质形成新蛋白质的生成力。我们报告的异型性在N-末端区域的成熟生长/分化因子5（GDF 5）蛋白质的进化过程中发生的硬骨鱼。GDF 5的N-末端长度的变化被发现之间的硬骨鱼科间和目间，但没有硬骨鱼家庭或四足动物之间。我们进一步表明，脯氨酸和谷氨酰胺的成熟GDF 5的N-末端区域的增加发生在Eurypalii，更高的谱系硬骨鱼。因为碱性氨基酸，被认为是控制扩散，在该地区的所有物种的检查是保守的，我们认为，在进化过程中的成熟GDF 5蛋白的N-末端延长改变了蛋白质扩散在Eurypalii，导致高浓度的蛋白质在咽骨关节，软骨形成的位置在发展过程中。
Heterotypy is now recognized as a generative force in the formation of new proteins through modification of existing proteins. We report that heterotypy in the N-terminal region of the mature growth/differentiation factor 5 (GDF5) protein occurred during evolution of teleosts. N-terminal length variation of GDF5 was found among teleost interfamilies and interorders but not within teleost families or among tetrapods. We further show that increase of proline and glutamine to the N-terminal region of mature GDF5 occurred in Eurypterygii, the higher lineage of teleosts. Because the basic amino acids, believed to control diffusion, are conserved in this region across all species examined, we suggest that the N-terminal elongation of the mature GDF5 protein during evolution has altered the protein diffusion in Eurypterygii, leading to high concentrations of the protein in the joint of the pharyngeal skeleton, the location of cartilage formation during development.