Variations of bacterial community structure in flooded paddy soil contaminated with herbicide quinclorac
Variations of bacterial community structure in flooded paddy soil contaminated with herbicide quinclorac
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DOI：
10.1080/03601230600805873
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发表时间：
2006-01-01
影响因子：
2
通讯作者：
Ye, Yangfang
中科院分区：
文献类型：
--
作者：
Lu, Zhenmei;Min, Hang;Ye, Yangfang
采用变性梯度凝胶电泳（DGGE）技术，研究了施用除草剂二氯喹啉酸（3，7-dichloro-8-quinoline-carboxylic acid，3，7-dichloro-8-quinoline-carboxylic acid）后淹水稻田土壤微生物群落的相对遗传复杂性。结果表明，二氯喹啉酸对不同浓度的除草剂污染的土壤中细菌种群的发展有显着影响。但总体而言，在前8周内，随着培养时间的延长，同一土壤样品经相同浓度的二氯喹啉酸处理后的种群数量差异明显。带型差异的规模表明，二氯喹啉酸处理和非二氯喹啉酸处理的土壤中的微生物群落结构没有显着差异后，培养21周。如本文所述，通过建立剂量-反应关系进行了定量研究。量化了显著的模式变化。切下突出的DGGE条带，克隆并测序以深入了解主要细菌群体的身份。DGGE条带序列主要与梭菌属、鞘氨醇杆菌属、黄单胞菌属和红球菌属有关。
The denaturing gradient gel electrophoresis (DGGE) method was applied to determine the relative genetic complexity of microbial communities in flooded paddy soil treated with herbicide quinclorac (3,7-dichloro-8-quinoline-carboylic acid). The results obtained showed a significant effect of quinclorac on the development of bacterial populations in soils contaminated with different concentrations of the herbicide at the early time after application. In general, however, the number of populations of the same soil sample treated with the same concentration of the quinclorac differed obviously with increasing incubation time within the early 8 weeks. The scale of differences in banding patterns-showed that the microbial community structures of the quinclorac-treated and non-quinclorac-treated soils were not significantly different after 21 weeks of incubation. Quantification, as demonstrated in this paper, was studied by establishing dose-response relationships. Significant pattern variations were quantified. Prominent DGGE bands were excised, cloned and sequenced to gain insight into the identities of predominant bacterial populations. The majority of DGGE band sequences were related to bacterial genera Clostridium, Sphingobacterium, Xanthomonas and Rhodococcus.