Ceramides mediate positional signals in Arabidopsis thaliana protoderm differentiation
Ceramides mediate positional signals in Arabidopsis thaliana protoderm differentiation
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DOI：
10.1242/dev.194969
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发表时间：
2021-01-01
期刊：
影响因子：
4.6
通讯作者：
Abe, Mitsutomo
中科院分区：
文献类型：
--
作者：
Nagata, Kenji;Ishikawa, Toshiki;Abe, Mitsutomo
不同细胞类型以适当模式的分化是多细胞生物体发育的基本过程。在拟南芥（Arabidopsis thaliana）中，原胚层/表皮在最外位置分化为单细胞层。然而，很少有人知道的分子性质的位置信号，实现正确的表皮细胞分化。在这里，我们提出，verylong-chain脂肪酸含神经酰胺（VLCFA-Cers）介导的位置信号刺激功能的拟南芥茎层1（ATML 1），一个主调节原皮层/表皮分化，侧根发育过程中。我们表明，VLCFA-Cers，主要是在最外层的细胞中合成，结合到ATML 1的脂质结合结构域。重要的是，这种细胞类型特异性蛋白质-脂质结合改变了ATML 1蛋白的活性，从而通过转录反馈环将其表达限制在原皮/表皮。此外，在面向外部环境的外侧膜处建立富含含有VLCFA的鞘脂的隔室可能起着决定原胚层细胞命运的作用。综上所述，我们的研究结果表明VLCFA-Cers通过介导ATML 1的位置信号在指导原皮/表皮分化中起着关键作用。
The differentiation of distinct cell types in appropriate patterns is a fundamental process in the development of multicellular organisms. In Arabidopsis thaliana, protoderm/epidermis differentiates as a single cell layer at the outermost position. However, little is known about the molecular nature of the positional signals that achieve correct epidermal cell differentiation. Here, we propose that verylong-chain fatty acid-containing ceramides (VLCFA-Cers) mediate positional signals by stimulating the function of ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1), a master regulator of protoderm/epidermis differentiation, during lateral root development. We show that VLCFA-Cers, which are synthesized predominantly in the outermost cells, bind to the lipid-binding domain of ATML1. Importantly, this cell type-specific protein-lipid association alters the activity of ATML1 protein and consequently restricts its expression to the protoderm/epidermis through a transcriptional feedback loop. Furthermore, establishment of a compartment, enriched with VLCFA-containing sphingolipids, at the outer lateral membrane facing the external environment may function as a determinant of protodermal cell fate. Taken together, our results indicate that VLCFA-Cers play a pivotal role in directing protoderm/epidermis differentiation by mediating positional signals to ATML1.This article has an associated The people behind the papers' interview.