ESTROGEN-BINDING SITES IN HAMSTER WHITE ADIPOSE-TISSUE - SEX-RELATED AND SITE-RELATED VARIATIONS - MODULATION BY TESTOSTERONE
ESTROGEN-BINDING SITES IN HAMSTER WHITE ADIPOSE-TISSUE - SEX-RELATED AND SITE-RELATED VARIATIONS - MODULATION BY TESTOSTERONE
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DOI：
10.1006/gcen.1995.1147
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发表时间：
1995-11-01
影响因子：
2.7
通讯作者：
GIUDICELLI, Y
中科院分区：
文献类型：
--
作者：
JAUBERT, AM;PECQUERY, R;GIUDICELLI, Y
以17 β -[H-3]雌二醇为配体，研究了雄性和雌性仓鼠附睾、准体和皮下脂肪组织中雌激素结合位点（ER）的变化。与其他哺乳动物脂肪沉积相比，完整的雄性和雌性仓鼠具有丰富的雌激素结合位点，对雌二醇具有中等亲和力，在雄性中为一类受体，而在雌性中为两个群体。雌激素受体的水平取决于性别和组织定位。在男性中，与女性相比，这两个部位的受体密度更高，ER在浅层脂肪沉积物中比在深层脂肪组织中更丰富。在女性中，有两个结合雌激素的群体；与雄性仓鼠相比，亲和性最高的一个与经典雌激素受体相似，两个种群的深层脂肪都比皮下沉积物丰富。这些特征取决于雄激素状态：在男性脂肪组织中，睾丸激素（TP）上调内质网水平。相反，在雌性脂肪沉积中，TP下调亲和性最高的雌激素受体，亲和性最低的群体消失。结合亲合力从不受睾酮的影响。这些结果表明，在仓鼠脂肪组织中，雌激素受体表现出与部位和性别相关的差异，正如先前对雄激素受体的描述。此外，雌性激素受体的表达受雄性激素状态的调节，这可能与在仓鼠中观察到的一些生理情况有关。(C) 1995学术出版社，Inc。
Estrogen binding sites (ER) were studied, using 17 beta-[H-3]estradiol as the ligand, in epididymal or parametrial and subcutaneous adipose tissues of male and female hamsters. Compared with other mammalian fat deposits, intact male and female hamsters possess abundant estrogen binding sites with moderate affinity for estradiol and which occur as a single class of receptor in males but as two populations in females. The levels of estrogen receptors depend on both sex and tissue localization. In males, receptor densities are higher in both localizations when compared to those of females and ER are more abundant in superficial adipose deposits than in the deep fat tissue. In females, there are two estrogen binding populations; the one with the highest affinity is similar to the classical estrogen receptor and both populations are more abundant in deep fat than in subcutaneous deposits, in contrast to male hamsters. These characteristics depend on androgen status: in male adipose tissues, testosterone (TP) up-regulates the ER levels. Conversely, in female fat deposits, TP down-regulates the highest affinity estrogen receptors and the lowest affinity population disappears. Binding affinities are never affected by testosterone. These results suggest that, in hamster adipose tissue, estrogen receptors exhibit site- and sex-related differences, as previously described for androgen receptors. Furthermore, estrogen receptor expression is modulated by the androgen status, depending on gender, which could be related to some physiological situations observed in the hamster. (C) 1995 Academic Press, Inc.