A stable sandwich-type amperometric biosensor based on poly(3,4-ethylenedioxythiophene)–single walled carbon nanotubes/ascorbate oxidase/nafion films for detection of L-ascorbic acid
A stable sandwich-type amperometric biosensor based on poly(3,4-ethylenedioxythiophene)–single walled carbon nanotubes/ascorbate oxidase/nafion films for detection of L-ascorbic acid
复制标题
DOI：
10.1016/j.snb.2011.07.005
复制
发表时间：
2011-11
影响因子：
8.4
通讯作者：
M. Liu;Yangping Wen;Dong Li;Ruirui Yue;Jingkun Xu;Haohua He
中科院分区：
文献类型：
--
作者：
M. Liu;Yangping Wen;Dong Li;Ruirui Yue;Jingkun Xu;Haohua He
本文成功研制了一种基于聚（3,4-乙烯二氧噻吩）(PEDOT) -单壁碳纳米管(SWCNT)/抗坏血酸氧化酶(AO)/Nafion薄膜的稳定型三明治型抗坏血酸（AA）生物传感器。内膜采用pedot - swcnts纳米复合材料，外膜采用Nafion膜。AO被固定在这两层薄膜之间。采用电化学阻抗谱和扫描电镜对PEDOT-SWCNT纳米复合膜进行了表征。详细考察了检测电位和温度对传感器性能的影响。尽管采用多层结构，但该传感器的响应速度相对较快（小于10s），线性范围为1μM ~ 18mM（相关系数为0.9974）。该生物传感器的灵敏度为28.5mAM−1cm−2。其实验检出限为0.7μM (S/N=3)，表观Michaelis-Menten常数（Km）为18.35mM。此外，该传感器具有良好的抗干扰能力和良好的长期稳定性。这些结果表明，这种三明治型pedot - swcnts /AO/Nafion薄膜可以为AA检测的生物传感器设计提供一个有前途的平台。
In this paper, a stable sandwich-type amperometric biosensor based on poly(3,4-ethylenedioxythiophene) (PEDOT)–single walled carbon nanotubes (SWCNT)/ascorbate oxidase (AO)/Nafion films for detection of l-ascorbic acid (AA) was successfully developed. PEDOT–SWCNT nanocomposite and Nafion films were used as inner and outer films, respectively. AO was immobilized between these two films. The PEDOT–SWCNT nanocomposite films were characterized by electrochemical impedance spectroscopy and scanning electron microscopy. The influence of detection potential and temperature on the biosensor performance was examined in detail. Despite the multilayer configuration, the biosensor exhibited a relatively fast response (less than 10s) and a linear range from 1μM to 18mM (a correlation coefficient of 0.9974). The sensitivity of the biosensor was found to be 28.5mAM−1cm−2. Its experimental detection limit was 0.7μM (S/N=3) and the apparent Michaelis–Menten constant (Km) was calculated to be 18.35mM. Moreover, the biosensor exhibited good anti-interferent ability and excellent long-term stability. All the results showed that such sandwich-type PEDOT–SWCNT/AO/Nafion films could provide a promising platform for the biosensor designs for AA detection.