Correlation of a novel matrix protein with the degree of cartilage degradation
Correlation of a novel matrix protein with the degree of cartilage degradation
复制标题
新型基质蛋白与软骨退化程度的相关性
DOI：
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发表时间：
2002
影响因子：
4
通讯作者：
A. Bosserhoff
中科院分区：
文献类型：
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作者：
B. Schmidt‐Rohlfing;U. Schneider;M. Thomsen;A. Bosserhoff
摘要软骨来源的维甲酸敏感蛋白(CD-RAP)，又称黑色素瘤抑制活性(MIA)，仅在软骨组织中生理性表达，在恶性黑色素瘤中有病理表达。最近的研究表明，这种蛋白可能是关节新陈代谢改变或损伤的有用标志。这项研究的目的是评估软骨退化程度与该蛋白水平之间是否存在相关性。在一项前瞻性横断面研究中，从67名连续接受膝关节手术的患者中采集了滑液样本。术中软骨退化程度采用Outerbridge和Noyes分类进行判定。MIA/CD-RAP采用商品化的酶联免疫吸附试验(EL ISA)测定。将标记水平与Outerbridge和Noyes分类进行比较，并计算相关系数。在软骨降解的晚期，滑液中MIA/CD-RAP水平降低。Oterbridge和Noyes分类的相关系数分别为-0.449和-0.418，具有较大的95%可信区间。虽然MIA/CD-RAP的生理功能尚不清楚，但结果表明，MIA/CD-RAP的水平取决于软骨降解的程度。尽管个体间差异很大，MIA/CD-RAP仍可作为监测退行性关节病变的标志物。
Abstract Cartilage-derived retinoic acid-sensitive protein (CD-RAP), also referred to as melanoma-inhibiting activity (MIA), is detected physiologically only in cartilage tissue and pathologically in malignant melanoma. Recent studies indicated that this protein might be useful as a marker for altered joint metabolism or damage. The purpose of this study was to evaluate whether there is any correlation between the degree of cartilage degradation and levels of this protein. In a prospective cross-sectional study, synovial fluid samples were obtained from 67 consecutive patients undergoing surgery of the knee joint. The degree of cartilage degradation was determined intraoperatively using the Outerbridge and Noyes classifications. MIA/CD-RAP was measured by a commercially available enzyme-linked immunosorbent assay (ELISA). The marker levels were compared against the Outerbridge and Noyes classifications and the correlation coefficients were calculated. At advanced stages of cartilage degradation, there were decreased synovial fluid levels of MIA/CD-RAP. The correlation coefficients were –0.449 and –0.418 for the Outerbridge and Noyes classifications, respectively, with large 95% confidence intervals. While the physiological function of MIA/CD-RAP is still unclear, the results indicate that MIA/CD-RAP levels depend on the degree of cartilage degradation. Despite the high interindividual variations, MIA/CD-RAP might be useful as a marker to monitor degenerative joint lesions.