Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology
Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology
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DOI：
10.1021/acsphotonics.3c01570
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发表时间：
2024-02-03
期刊：
影响因子：
7
通讯作者：
Vollmer，Frank
中科院分区：
文献类型：
--
作者：
Houghton，Matthew C.;Kashanian，Samir Vartabi;Vollmer，Frank
光学微腔，特别是回音壁模式（WGM）微腔，由于其对环境变化的显着敏感性，已被广泛用作生物传感器，使检测范围广泛的生物分子和纳米颗粒。为了将检测极限降低到最灵敏的单分子水平，等离子体纳米棒被战略性地引入以增强WGM微腔的倏逝场。光等离子体WGM传感器的这一进步允许检测蛋白质的单个分子、构象变化甚至原子离子，标志着单分子传感的重大贡献。该展望讨论了光等离子体WGM单分子传感的令人兴奋的研究前景，包括酶的热力学和动力学研究，热光等离子体传感的出现，WGM微激光器的超灵敏单分子传感，以及在合成生物学中的应用。
Optical microcavities, specifically, whispering-gallery mode (WGM) microcavities, with their remarkable sensitivity to environmental changes, have been extensively employed as biosensors, enabling the detection of a wide range of biomolecules and nanoparticles. To push the limits of detection down to the most sensitive single-molecule level, plasmonic nanorods are strategically introduced to enhance the evanescent fields of WGM microcavities. This advancement of optoplasmonic WGM sensors allows for the detection of single molecules of a protein, conformational changes, and even atomic ions, marking significant contributions in single-molecule sensing. This Perspective discusses the exciting research prospects in optoplasmonic WGM sensing of single molecules, including the study of enzyme thermodynamics and kinetics, the emergence of thermo-optoplasmonic sensing, the ultrasensitive single-molecule sensing on WGM microlasers, and applications in synthetic biology.