Effects of baicalein isolated from Scutellaria baicalensis Radix on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide in cultured human umbilical vein endothelial cells.
Effects of baicalein isolated from Scutellaria baicalensis Radix on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide in cultured human umbilical vein endothelial cells.
复制标题
黄芩黄芩素对凝血酶和凝血酶受体激动肽诱导的人脐静脉内皮细胞粘附分子表达的影响。
作者：
Y. Kimura;N. Matsushita;K. Yokoi;H. Okuda
本实验研究了黄芩不同黄酮类化合物对凝血酶及凝血酶受体激动肽（SFLLRNPNDKYEPF， TRAP）诱导的人脐静脉内皮细胞粘附分子表达的影响。凝血酶和凝血酶受体激动剂肽诱导内皮白细胞粘附分子-1 （ELAM-1）表达。凝血酶也能诱导细胞间粘附分子-1 （ICAM-1）的表达，而TRAP则不能。黄芩苷对凝血酶和凝血酶受体激动剂肽诱导的ELAM-1表达具有剂量依赖性，50%抑制浓度（IC50）分别为5.53 +/- 1.68 μ m和2.44 +/- 1.08 μ m。此外，黄芩素抑制凝血素诱导的ICAM-1表达，IC50为9.67 +/- 1.28微米。此外，黄芩素还能抑制蛋白激酶C （PKC）激活剂肉豆蔻酸酯phorbol acetate （PMA）刺激的ELAM-1和ICAM-1表达。
We examined the effects of various flavonoids isolated from the roots of Scutellaria baicalensis Georgi on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide (SFLLRNPNDKYEPF, TRAP) in cultured human umbilical vein endothelial cells. Thrombin and thrombin receptor agonist peptide induced endothelial leukocyte adhesion molecule-1 (ELAM-1) expression. Intercellular adhesion molecule-1 (ICAM-1) expression was also induced by thrombin, but not by TRAP. Baicalein isolated from Scutellariae Radix inhibited ELAM-1 expression induced by thrombin and thrombin receptor agonist peptide dose-dependently, with 50% inhibitory concentrations (IC50) of 5.53 +/- 1.68 microM and 2.44 +/- 1.08 microM, respectively. Furthermore, baicalein inhibited thrombin-induced ICAM-1 expression with an IC50 of 9.67 +/- 1.28 microM. In addition, baicalein inhibited the expressions of ELAM-1 and ICAM-1 stimulated by protein kinase C (PKC) activator phorbol myristate acetate (PMA).