An in vitro model of epithelial cell growth stimulation in the rodent mammary gland
An in vitro model of epithelial cell growth stimulation in the rodent mammary gland
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DOI：
10.1046/j.1365-2184.2003.00265.x
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发表时间：
2003-08-01
影响因子：
8.5
通讯作者：
Omary, MB
中科院分区：
文献类型：
--
作者：
Ehmann, UK;DeVries, JT;Omary, MB
先前描述的小鼠乳腺上皮细胞培养物引起广泛增殖和具有适当的管腔导管上皮细胞标记的细胞群，以及在植入小鼠后形成正常组织的能力。这种成功可能是由于培养环境类似于乳腺中环境的某些方面。小鼠乳腺上皮细胞，其增殖是有限的，当单独铺板时，可以通过与LA 7大鼠乳腺肿瘤系的致死性辐照细胞接触刺激增殖。大部分的增殖刺激是通过LA 7和小鼠乳腺细胞之间的直接细胞接触来传递的。连接，包括粘附连接，在培养物中的所有细胞之间形成，就像在乳腺中形成的连接一样。LA 7细胞分泌TGF α和bFGF，这是在乳腺中发现的因子，以及小鼠乳腺细胞在培养物中响应的因子。小鼠乳腺细胞表达角蛋白8和18，乳腺导管腔细胞的标记物。LA 7细胞表达角蛋白14和波形蛋白，肌上皮细胞的标志物。这些事实结合在一起，符合上皮组织中细胞替代的模型，并且还模仿了乳腺中管腔导管细胞和肌上皮细胞之间的关系。这种培养细胞的方法不仅可用于体外-体内致癌研究，而且可用于研究生长信号从一个细胞传递到另一个细胞的机制。
Mouse mammary epithelial cell cultures previously described bring about extensive proliferation and a cell population with the appropriate markers for luminal ductal epithelial cells, and also the ability to form normal tissue after implantation into mice. This success may result from a culture environment that resembles certain aspects of the environment in the mammary gland. Mouse mammary epithelial cells, whose proliferation is limited when plated alone, can be stimulated to multiply by contact with lethally irradiated cells of the LA7 rat mammary tumour line. Most of the proliferative stimulus is imparted by direct cell contact between LA7 and mouse mammary cells. Junctions, including adherens junctions, form among all cells in the culture, much as junctions form in the mammary gland. LA7 cells secrete TGFalpha and bFGF, factors found in the mammary gland, and factors to which mouse mammary cells respond in culture. Mouse mammary cells express keratins 8 and 18, markers for luminal cells of the mammary duct. LA7 cells express keratin 14 and vimentin, markers for myoepithelial cells. These facts, taken together, fit a model of cell replacement in an epithelial tissue and also imitate the relationship between luminal ductal cells and myoepithelial cells in the mammary gland. This method of culturing cells is useful, not only for in vitro-in vivo carcinogenesis studies, but also for the study of mechanisms by which growth signals are imparted from one cell to another.