Characterization of Bordetella pertussis growing as biofilm by chemical analysis and FT-IR spectroscopy
Characterization of Bordetella pertussis growing as biofilm by chemical analysis and FT-IR spectroscopy
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DOI：
10.1007/s00253-005-0202-8
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发表时间：
2006-08-01
影响因子：
5
通讯作者：
Yantorno, O.
中科院分区：
文献类型：
--
作者：
Bosch, A.;Serra, D.;Yantorno, O.
虽然百日咳博德特氏菌是百日咳的病原体，它附着并生长在呼吸道的纤毛上皮上，但仅在液体培养中进行了广泛的研究。本研究对B.生物膜生长中的百日咳被描述为在宿主定殖中可能发生的事件的第一近似。通过化学方法和傅里叶变换红外光谱（FT-IR）监测聚丙烯珠上的生物膜。细胞包膜的分析显示，外膜蛋白（OMP）模式和脂多糖（LPS）的表达在生物膜中的无变化的差异最小，与无张力生长的细胞相比。无柄细胞表现出2.4- 3.0倍的碳水化合物/蛋白质的比例相比，不同类型的线粒体细胞。观察到多糖含量增加1.8倍，亲水性显著增加。FT-IR光谱的生物膜细胞显示更高的强度，在吸收带分配给多糖（1200 -900 cm（-1）区域）和振动模式的羧酸基团（1,627，1,405，和1,373 cm（-1））相比，光谱的质子化细胞。在生物膜基质中，检测到含糖醛酸的多糖、蛋白质和LPS。生物膜生长过程中胞外碳水化合物的产生与特定生长速率、生长阶段或氧限制的变化无关。它可能代表了一个额外的毒力因子，可能有助于B。逃避宿主的防御
Although Bordetella pertussis, the etiologic agent of whooping cough, adheres and grows on the ciliated epithelium of the respiratory tract, it has been extensively studied only in liquid cultures. In this work, the phenotypic expression of B. pertussis in biofilm growth is described as a first approximation of events that may occur in the colonization of the host. The biofilm developed on polypropylene beads was monitored by chemical methods and Fourier transform infrared (FT-IR) spectroscopy. Analysis of cell envelopes revealed minimal differences in outer membrane protein (OMP) pattern and no variation of lipopolysaccharide (LPS) expression in biofilm compared with planktonically grown cells. Sessile cells exhibited a 2.4- to 3.0-fold higher carbohydrate/protein ratio compared with different types of planktonic cells. A 1.8-fold increased polysaccharide content with significantly increased hydrophilic characteristics was observed. FT-IR spectra of the biofilm cells showed higher intensity in the absorption bands assigned to polysaccharides (1,200-900 cm(-1) region) and vibrational modes of carboxylate groups (1,627, 1,405, and 1,373 cm(-1)) compared with the spectra of planktonic cells. In the biofilm matrix, uronic-acid-containing polysaccharides, proteins, and LPS were detected. The production of extracellular carbohydrates during biofilm growth was not associated with changes in the specific growth rate, growth phase, or oxygen limitation. It could represent an additional virulence factor that may help B. pertussis to evade host defenses.