8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols as the marker compounds of Chinese dark teas formed in the post-fermentation process provide significant antioxidative activity
8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols as the marker compounds of Chinese dark teas formed in the post-fermentation process provide significant antioxidative activity
复制标题
8-C N-乙基-2-吡咯烷酮取代的黄烷-3-醇作为中国红茶在发酵后过程中形成的标记化合物，具有显着的抗氧化活性
DOI：
10.1016/j.foodchem.2013.10.117
复制
发表时间：
2014-06-01
期刊：
影响因子：
8.8
通讯作者：
Tu, Pengfei
中科院分区：
文献类型：
--
作者：
Wang, Weinan;Zhang, Liang;Tu, Pengfei
通过H-1、C-13、二维核磁共振（NMR）和高效液相色谱-二极管阵列检测器-电喷雾电离质谱（HPLC-DAD-ESI/MS）分析，从普洱茶水提物中分离得到8个新的8-C N-乙基-2-吡咯烷酮取代黄烷-3-醇（葛根素1-VIII）。通过对绿色茶、红茶和中国黑茶的比较化学分析，证实这些化合物是中国黑茶的标志化合物。此外，真菌发酵对于这些新化合物的生物合成是必不可少的。通过单菌发酵，证明了儿茶素和茶氨酸是葛根素的前体物质。HPLC-DAD-ESI/MS分析阐明了葛根素的生物合成途径。与其他茶多酚相比，葛根素I-IV对过氧化氢诱导的人微血管内皮细胞（HMEC）损伤具有潜在的保护作用。8-C-N-乙基-2-吡咯烷酮取代黄烷-3-醇可用于黑茶的质量控制和鉴别。(C)2013爱思唯尔有限公司保留所有权利。
Phytochemical investigation of the aqueous extract of pu-erh tea afforded eight novel 8-C N-ethy1-2-pyrrolidinone substituted flavan-3-ols (puerins 1-VIII) by H-1, C-13, two-dimensional nuclear magnetic resonance (NMR) and high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS) analysis. Comparative chemical analysis of green tea, black tea and Chinese dark teas confirmed that these compounds were the marker compounds of Chinese dark teas. Furthermore, fungal fermentation was indispensable for the biosynthesis of these novel compounds. Through single fungal fermentation, it was proved that catechins and theanine were the precursors of puerins HPLC-DAD-ESI/MS analysis elucidated the biosynthetic pathway for puerins Puerins I-IV have potential protective effects for the human micro-vascular endothelial cells (HMEC) injury induced by hydrogen dioxide compared to other tea polyphenols. 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols could be used in the quality control and authentication of Chinese dark teas. (C) 2013 Elsevier Ltd. All rights reserved.