Sequence analysis of conserved regA and variable orf43.1 genes in T4-like bacteriophages.
Sequence analysis of conserved regA and variable orf43.1 genes in T4-like bacteriophages.
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T4 样噬菌体中保守 regA 和可变 orf43.1 基因的序列分析。
DOI：
10.1128/jb.172.9.5180-5186.1990
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发表时间：
1990
影响因子：
3.2
通讯作者：
Jozwik,CE
中科院分区：
文献类型：
--
作者：
Miller,ES;Jozwik,CE
噬菌体T4 RegA蛋白是几种噬菌体mrna的翻译抑制因子。在检测的t4相关噬菌体中，regA核苷酸序列高度保守，推断的氨基酸序列相同。这种特殊的噬菌体RB69不能通过免疫印迹（Western blots）产生可重复识别的RegA蛋白，也不能产生与T4 RegA引物杂交的mRNA。在RB18和RB51中立即鉴定出223或250个碱基对的3′- regA核苷酸序列，这些序列在T-even噬菌体中缺失。这两个区域的开放阅读框分别被命名为orf43.1RB18和orf43.1RB51，可能分别编码8.5千道尔顿和9.2千道尔顿的相关蛋白。聚合酶链反应分析的27条RB噬菌体染色体中，有13条检测到orf43.1序列为RB18或rb51样，其侧翼重复序列可能促进orf43.1的缺失。
Bacteriophage T4 RegA protein is a translational repressor of several phage mRNAs. In the T4-related phages examined, regA nucleotide sequences are highly conserved and the inferred amino acid sequences are identical. The exceptional phage, RB69, did not produce a RegA protein reproducibly identifiable by Western blots (immunoblots) nor did it produce mRNA that hybridized to T4 regA primers. Nucleotide sequences of either 223 or 250 base pairs were identified immediately 3' to regA in RB18 and RB51 that were absent in T-even phages. Open reading frames in these regions, designated orf43.1RB18 and orf43.1RB51, potentially encode related proteins of 8.5 and 9.2 kilodaltons, respectively. orf43.1 sequences, detected in 13 of 27 RB bacteriophage chromosomes analyzed by polymerase chain reaction, are either RB18- or RB51-like and have flanking repeat sequences that may promote orf43.1 deletion.