In vivo fluorescence of the ocular fundus exhibits retinal pigment epithelium lipofuscin characteristics.
In vivo fluorescence of the ocular fundus exhibits retinal pigment epithelium lipofuscin characteristics.
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发表时间：
1995-03
影响因子：
4.4
通讯作者：
F. Delori;C. Dorey;G. Staurenghi;O. Arend;D. Goger;J. Weiter
中科院分区：
文献类型：
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作者：
F. Delori;C. Dorey;G. Staurenghi;O. Arend;D. Goger;J. Weiter
目的从激发和发射光谱、年龄关系、视网膜位置和形态学等方面对人眼底的内源性荧光（自发荧光）进行表征，并确定眼底各层中的主要荧光团。方法采用新型眼底分光光度计，对30例正常人和3例经筛选的患者进行了中心凹颞侧7 °和中心凹荧光测量。对3名受试者进行了地形测量。获得人视网膜色素上皮（RPE）的荧光的离体测量，并与体内数据进行比较。结果眼底荧光显示在500和750 nm之间的宽发射带，最大值约为630 nm，最佳激发波长约为510 nm。随着年龄的增长表现出显着的增加，这种荧光是最高的在7度至15度的中心凹，显示出一个明确的中心凹最小值，并朝着周边减少。体内荧光光谱与离体人RPE上获得的荧光光谱一致。用短波长激发的测量强烈地受眼部介质吸收的影响，并揭示了在中央凹中的额外的次要荧光团。结论：脉络膜毛细血管与感光细胞之间的光谱特征、年龄相关性、地形分布和视网膜位置表明，眼底主要荧光团是RPE脂褐素。次要的荧光团可能是在神经视网膜，但尚未确定。
PURPOSE To characterize the intrinsic fluorescence (autofluorescence) of the human ocular fundus with regard to its excitation and emission spectra, age relationship, retinal location, and topography, and to identify the dominant fluorophore among the fundus layers. METHODS Using a novel fundus spectrophotometer, fluorescence measurements were made at 7 degrees temporal to the fovea and at the fovea in 30 normal subjects and in 3 selected patients. Topographic measurements were made in 3 subjects. Ex vivo measurements of fluorescence of human retinal pigment epithelium (RPE) were obtained and compared to in vivo data. RESULTS Fundus fluorescence reveals a broad band of emission between 500 and 750 nm, a maximum of approximately 630 nm, and optimal excitation of approximately 510 nm. Exhibiting a significant increase with age, this fluorescence is highest at 7 degrees to 15 degrees from the fovea, shows a well-defined foveal minimum, and decreases toward the periphery. In vivo fluorescence spectra are consistent with those obtained ex vivo on human RPE. Measurements with short wavelength excitation are strongly influenced by ocular media absorption and reveal an additional minor fluorophore in the fovea. CONCLUSIONS Spectral characteristics, correlation with age, topographic distribution, and retinal location between the choriocapillaris and the photoreceptors suggest that the dominant fundus fluorophore is RPE lipofuscin. The minor fluorophore is probably in the neurosensory retina but has not been identified.