Level of platelet-derived cytokines in leukoreduced red blood cells is influenced by the processing method and type of leukoreduction filter
Level of platelet-derived cytokines in leukoreduced red blood cells is influenced by the processing method and type of leukoreduction filter
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DOI：
10.1111/j.1537-2995.2009.02353.x
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发表时间：
2010-01-01
期刊：
影响因子：
2.9
通讯作者：
Sparrow, Rosemary L.
中科院分区：
文献类型：
--
作者：
Glenister, Kristen M.;Sparrow, Rosemary L.
背景：与白细胞对红细胞（rbc）质量的影响有充分的文献记载相反，血小板（PLTs）的影响很少得到考虑。在本研究中，研究了使用不同类型的白细胞诱导方法制备的红细胞中PLT的含量和PLT衍生细胞因子的水平。研究设计和方法：根据标准血库条件制备和保存无Buffy涂层红细胞和五种类型的白细胞滤过（LF）红细胞，包括用全血（WB） plt保存过滤器制备的红细胞。在第1天测量PLT含量，并在42天的存储期间的指定时间点通过酶联免疫吸附法测量PLT衍生细胞因子的水平。结果：白细胞诱导红细胞的PLT含量根据处理方法和/或使用的白细胞诱导过滤器而有很大差异，某些类型的红细胞的PLT计数非常低，而其他单位的PLT计数与未处理的白细胞相当。红细胞中PLT的含量直接影响PLT衍生细胞因子的浓度和积累。几个plt衍生因子在42天的储存中表现出显著的积累。具有高PLT含量的红细胞显示出与先前报道相当的RANTES （CCL5）和可溶性CD40配体浓度，显示出显著的生物学和临床效应。结论：白细胞诱导的红细胞中PLT含量和PLT来源的细胞因子水平受处理方法和白细胞诱导过滤器类型的影响。根据不同类型的白细胞诱导过滤器制备的lf -红细胞的PLT因子水平不同，将其视为等效产品可能是不合适的。
BACKGROUND:In contrast to the well-documented effect of white blood cells on the quality of red blood cells (RBCs), the effect of platelets (PLTs) has received little consideration. In this study, the PLT content and level of PLT-derived cytokines in RBCs prepared using different types of leukoreduction methods were investigated.STUDY DESIGN AND METHODS:Buffy coat-poor RBCs and five types of leukofiltered (LF) RBCs, including RBCs prepared with a whole blood (WB) PLT-saving filter, were prepared and stored according to standard blood bank conditions. PLT content was measured on Day 1, and levels of PLT-derived cytokines were measured by enzyme-linked immunosorbent assay at nominated timepoints during 42 days of storage.RESULTS:The PLT content of leukoreduced RBCs varied widely depending on the processing method and/or leukoreduction filter used, with some types of RBCs containing very low PLT counts while other units contained PLT counts comparable to those of unprocessed WB. The PLT content of RBCs directly influenced the concentration and accumulation of PLT-derived cytokines. Several PLT-derived factors exhibited significant accumulation throughout 42 days of storage. RBCs with high PLT content exhibited concentrations of RANTES (CCL5) and soluble CD40 ligand equivalent to those previously reported to show significant biologic and clinical effects.CONCLUSION:The PLT content and levels of PLT-derived cytokines in leukoreduced RBCs are influenced by the processing method and types of leukoreduction filters used. It may be inappropriate to consider LF-RBCs prepared with different types of leukoreduction filters as equivalent products based on their differing levels of PLT factors.