Antibacterial Performance of TiCaPCON Films Incorporated with Ag, Pt, and Zn: Bactericidal Ions Versus Surface Microgalvanic Interactions.
Antibacterial Performance of TiCaPCON Films Incorporated with Ag, Pt, and Zn: Bactericidal Ions Versus Surface Microgalvanic Interactions.
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DOI：
10.1021/acsami.8b06671
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发表时间：
2018-07
影响因子：
9.5
通讯作者：
Viktor A Ponomarev;I. Sukhorukova;A. Sheveyko;E. Permyakova;A. Manakhov;Sergei G. Ignatov;N. Gloushankova;I. Zhitnyak;Oleg Igorevich Lebedev;J. Polčak;A. Kozmin;D. Shtansky
中科院分区：
文献类型：
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作者：
Viktor A Ponomarev;I. Sukhorukova;A. Sheveyko;E. Permyakova;A. Manakhov;Sergei G. Ignatov;N. Gloushankova;I. Zhitnyak;Oleg Igorevich Lebedev;J. Polčak;A. Kozmin;D. Shtansky
术后早期预防细菌定植非常重要。有四种主要的策略及其相应类型的抗菌表面专门设计用于抗感染：杀菌剂释放、抗粘连、pH敏感和接触杀灭。在此，我们旨在确定不同类型杀菌离子的抗菌效率，并揭示异质表面上电位差引起的表面微电流效应的可能贡献。我们考虑了五种类型的TiCaPCON薄膜，在它们的表面上具有Ag、Zn、Pt、Ag + Zn和Pt + Zn纳米颗粒（NP）。Ag改性的膜在生理溶液中0.11ppb的非常低的Ag离子浓度下显示出显著的抗菌效果，这在浸入大肠杆菌（Escherichia coli）中3小时后已经达到。coli）细菌培养物。含锌样品对大肠杆菌也有明显的抑菌效果。大肠杆菌和金黄色葡萄球菌（S.其中Zn离子的浓度比Ag离子的浓度高2个数量级（15 ppb）。Ag纳米粒子的存在加速了Zn离子从TiCaPCON-Ag-Zn膜中的浸出，但没有观察到两种杀菌组分同时存在的协同效应。将样品与Ag、Zn和Ag + Zn纳米颗粒在E. coli和革兰氏阳性菌S.金黄色葡萄球菌悬液分别作用24和8小时，所有细菌细胞完全灭活。含Pt的膜显示出非常低的Pt离子释放，因此这种类型的离子对总杀菌效果的贡献可以忽略。电化学测试和Kelvin探针力显微镜分析结果表明，Pt纳米颗粒与TiCaPCON薄膜之间形成了微电偶，但对大肠杆菌和铜绿假单胞菌均无明显的抗菌作用。coli或S.观察到金黄色葡萄球菌。所有离子改性样品均提供良好的成骨细胞附着、铺展和增殖，因此得出结论，对细胞无毒。此外，在TiCaPCON薄膜的表面分别沉积Ag、Pt和Zn纳米颗粒，均表现出良好的骨传导特性。
It is very important to prevent bacterial colonization at the early postoperative stages. There are four major strategies and their corresponding types of antibacterial surfaces specifically designed to fight infection: bactericide release, anti-adhesion, pH-sensitive, and contact-killing. Herein, we aimed at determining the antibacterial efficiency of different types of bactericidal ions and revealing the possible contribution of surface microgalvanic effects arising from a potential difference on heterogeneous surfaces. We considered five types of TiCaPCON films, with Ag, Zn, Pt, Ag + Zn, and Pt + Zn nanoparticles (NPs) on their surface. The Ag-modified film demonstrated a pronounced antibacterial effect at a very low Ag ion concentration of 0.11 ppb in physiological solution that was achieved already after 3 h of immersion in Escherichia coli ( E. coli) bacterial culture. The Zn-containing sample also showed a noticeable antibacterial effect against E. coli and Staphylococcus aureus ( S. aureus) strains, wherein the concentration of Zn ions was 2 orders of magnitude higher (15 ppb) compared with the Ag ions. The presence of Ag NPs accelerated the leaching of Zn ion out of the TiCaPCON-Ag-Zn film, but no synergistic effect of the simultaneous presence of the two bactericidal components was observed. After the incubation of the samples with Ag, Zn, and Ag + Zn NPs in E. coli and S. aureus suspensions for 24 and 8 h, respectively, all bacterial cells were completely inactivated. The Pt-containing film showed a very low Pt ion release, and therefore the contribution of this type of ions to the total bactericidal effect could be neglected. The results of the electrochemical studies and Kelvin probe force microscopy indicated that microgalvanic couples were formed between the Pt NPs and the TiCaPCON film, but no noticeable antibacterial effect against either E. coli or S. aureus strains was observed. All ion-modified samples provided good osteoblastic cell attachment, spreading, and proliferation and therefore were concluded to be nontoxic for cells. In addition, the TiCaPCON films with Ag, Pt, and Zn NPs on their surface demonstrated good osteoconductive characteristics.