Expression of stabilin-2, a novel fasciclin-like hyaluronan receptor protein, in murine sinusoidal endothelia, avascular tissues, and at solid/liquid interfaces
Expression of stabilin-2, a novel fasciclin-like hyaluronan receptor protein, in murine sinusoidal endothelia, avascular tissues, and at solid/liquid interfaces
复制标题
DOI：
10.1007/s00418-003-0585-5
复制
发表时间：
2003-11-01
影响因子：
2.3
通讯作者：
Goerdt, S
中科院分区：
文献类型：
--
作者：
Falkowski, M;Schledzewski, K;Goerdt, S
肝透明质酸受体Stabilin-2最近被我们克隆。与stabilin-1一起，stabilin-2构成了一个新的成束蛋白样透明质酸受体同系物家族。在这里，我们分析了稳定蛋白-2（mStab-2）在广泛的C57 BL/6小鼠器官和组织中的表达。虽然北方印迹分析显示mStab-2 mRNA仅在肝脏和脾脏中阳性表达，但免疫组织化学显示mStab-2蛋白在肝脏、淋巴结、脾脏和骨髓的内皮窦中以及眼、心脏、脑和肾脏的专门结构中表达。在角膜和透镜上皮细胞、心脏瓣膜的间充质细胞、衬于脑室的室管膜细胞和覆盖肾乳头的棱柱状上皮细胞中检测到mStab-2的表达。在病理条件下，如肿瘤生长或伤口愈合过程中，mStab-2在新形成的脉管系统或其他组织成分中不表达。基于这些结果，我们认为mStab-2可能参与通过内皮窦网络从淋巴或血液循环中清除透明质酸。在无血管和/或划分固/液界面的其他mStab-2阳性组织部位，mStab-2可能通过支持细胞外基质周转来维持组织完整性，或者可能通过吸收透明质酸来维持体液的流动性。
Stabilin-2, the hepatic hyaluronan receptor, has recently been cloned by us. Together with stabilin-1, stabilin-2 constitutes a novel family of fasciclin-like hyaluronan receptor homologues. Here, we analyzed expression of stabilin-2 (mStab-2) in a broad array of C57BL/6 mouse organs and tissues. While northern blot analysis showed positive expression of mStab-2 mRNA confined to liver and spleen, immunohistochemistry demonstrated mStab-2 protein expression in the endothelial sinuses of liver, lymph nodes, spleen, and bone marrow, and in specialized structures of eye, heart, brain, and kidney. Expression of mStab-2 was detected in corneal and lens epithelium, in mesenchymal cells of the heart valves, in the ependymal cells lining the ventricles in the brain, and in the prismatic epithelial cells covering the renal papillae. In pathological conditions, such as tumor growth or wound healing processes, mStab-2 was not expressed in the newly formed vasculature or other tissue components. Based on these results, we suggest that mStab-2 might be involved in the clearance of hyaluronan from the lymph or the blood circulation via the network of endothelial sinuses. At the other mStab-2-positive tissues sites that are either avascular and/or demarcate a solid/liquid interface, mStab-2 may serve to maintain tissue integrity by supporting extracellular matrix turnover or it may contribute to maintaining fluidity of bodily liquids by resorption of hyaluronan.