Evolution of the YABBY gene family with emphasis on the basal eudicot Eschscholzia californica (Papaveraceae)
Evolution of the YABBY gene family with emphasis on the basal eudicot Eschscholzia californica (Papaveraceae)
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DOI：
10.1111/j.1438-8677.2011.00486.x
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发表时间：
2012-01-01
期刊：
影响因子：
3.9
通讯作者：
Gleissberg, S.
中科院分区：
文献类型：
--
作者：
Bartholmes, C.;Hidalgo, O.;Gleissberg, S.
YABBY基因是种子植物特异性转录调控因子，参与了叶片、茎和花发育的各个方面。一系列的重复产生了在开花植物中发现的五个基因群。在拟南芥和其他物种中，螃蟹爪和INNER NO OUTER两个基因群的表达仅限于花器官。相反，丝状花、YABBY2和YABBY5基因群的成员也在叶片中表达，并被称为营养yabbyys。这五个平行类群是如何进化的，以及它们的表达和功能是如何多样化的，这在很大程度上仍未得到解决，因此无法重建这个基因家族的自然史。在这里，我们报告了来自加利福尼亚Eschscholzia （Ranunculales, Papaveraceae）的新基因，我们将这些基因与现有的数据库序列一起用于YABBY基因家族的全面系统发育重新评估。覆盖种子植物的多层贝叶斯分析使我们能够在基因家族系统发育中定位Eschscholzia YABBY序列。我们确定了营养的YABBYs不形成一个单系进化枝，螃蟹爪和丝状花起源于一个共同的祖先基因。内在基因和外在基因是祖先基因的姐妹。我们在已知氨基酸结构域之外鉴定了几个保守的基序，这些基序定义了所有五个被子植物YABBY基因分支。进一步，我们推断了基因表达的进化，并为基因家族树中某些分支的净化约束释放提供了证据。最后，我们报道了5个Eschscholzia YABBY基因的表达模式，这些基因在早期分化和核心分化之间的功能守恒是一致的。
YABBY genes are seed plant-specific transcriptional regulators that are involved in diverse aspects of leaf, shoot and flower development. A series of duplications gave rise to five gene groups found throughout flowering plants. In Arabidopsis and other species, expression of two gene groups, CRABS CLAW and INNER NO OUTER, is restricted to floral organs. In contrast, members of the FILAMENTOUS FLOWER, YABBY2 and YABBY5 gene groups are also expressed in leaves and have been termed vegetative YABBYs. How the five paralogue groups evolved and how their expression and function diversified have remained largely unresolved, precluding a reconstruction of the natural history of this gene family. Here, we report new genes from Eschscholzia californica (Ranunculales, Papaveraceae) that we use together with currently available database sequences in a comprehensive phylogenetic re-evaluation of the YABBY gene family. Multilayered Bayesian analysis covering seed plants allowed us to locate Eschscholzia YABBY sequences within the gene family phylogeny. We established that vegetative YABBYs do not form a monophyletic clade, and that CRABS CLAW and FILAMENTOUS FLOWER arose from a common ancestor gene. INNER NO OUTER genes are sister to that ancestral gene. We identified several conserved motifs outside of known amino acid domains that define all five angiosperm YABBY gene clades. Further, we inferred the evolution of gene expression and provide evidence for release of purifying constraint in certain branches of the gene family tree. Finally, we report expression patterns for five Eschscholzia YABBY genes consistent with functional conservation between early-diverged and core eudicots.