Acupuncture regulates the autophagy of ovarian granulosa cells in polycystic ovarian syndrome ovulation disorder by inhibiting the PI3K/AKT/mTOR pathway through LncMEG3
Acupuncture regulates the autophagy of ovarian granulosa cells in polycystic ovarian syndrome ovulation disorder by inhibiting the PI3K/AKT/mTOR pathway through LncMEG3
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DOI：
10.1016/j.biopha.2021.112288
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发表时间：
2021-10-13
影响因子：
7.5
通讯作者：
Lin, Jing
中科院分区：
文献类型：
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作者：
Chen, Xiaohong;Tang, Hongliang;Lin, Jing
多囊卵巢综合征（PCOS）的主要特征是卵泡发育异常和排卵功能障碍，这是由卵巢颗粒细胞过度自噬引起的。针灸已被证明可以改善 PCOS 患者的排卵功能障碍和卵泡发育异常，但其机制尚不清楚。本研究假设针灸的有益作用是 LncMEG3 介导的对 PI3K/AKT/mTOR 通路影响的结果。采用针灸（CV-4、RN-3、CV-6、SP-6 和 EX-CA 1）治疗多囊卵巢综合征大鼠模型。苏木精-伊红染色观察卵巢形态,酶联免疫吸附法、Western blotting、免疫组化、实时荧光定量PCR检测LH、E2、FSH、T、AMH、LncMEG3、PI3K、AKT、mTOR、P62、LC3II/I表达量。连续治疗11天后，针灸治疗组90%的大鼠卵巢形态明显改善。针灸还导致血清 LH、FSH、T 和 AMH 水平显着降低，E2 水平显着升高（P
The main features of polycystic ovary syndrome (PCOS) are abnormal follicular development and ovulation dysfunction, which are caused by the excessive autophagy of ovarian granulosa cells. Acupuncture has been shown to improve ovulation dysfunction and abnormal follicular development in PCOS patients, but its mechanism is unclear. This study hypothesized that the beneficial effects of acupuncture are the result of LncMEG3mediated effects on the PI3K/AKT/mTOR pathway. Acupuncture (CV-4, RN-3, CV-6, SP-6 and EX-CA 1) was used to treat a rat model of polycystic ovary syndrome. Hematoxylin-eosin staining was used to observe ovarian morphology and enzyme-linked immunosorbent assay, western blotting, immunohistochemistry and real-time PCR were used to detect LH, E2, FSH, T, AMH, LncMEG3, PI3K, AKT, mTOR, P62 and LC3II/I expression. The ovarian morphology of 90% of the rats in the acupuncture treatment group was significantly improved after 11 consecutive days of therapy. Acupuncture also resulted in a significant decrease in serum LH, FSH, T and AMH levels and a significant increase in E2 level (P