Oxysterols As Indices of Oxidative Stress in Man After Paraquat Ingestion
Oxysterols As Indices of Oxidative Stress in Man After Paraquat Ingestion
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DOI：
10.1080/10715760290006493
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发表时间：
2002-01
影响因子：
3.3
通讯作者：
K. Ishii;J. Adachi;M. Tomita;M. Kurosaka;Y. Ueno
中科院分区：
文献类型：
--
作者：
K. Ishii;J. Adachi;M. Tomita;M. Kurosaka;Y. Ueno
本研究的目的是通过分析7 f -和7 g -过氧化氢胆甾-5-烯-3 g -醇（7 f -和7 g -OOH）以及氧化固醇（胆固醇氧化产物）作为脂质过氧化的指标，评估摄入百草枯后人体的氧化应激。肺，肾脏和肝脏，收集在尸体解剖与百草枯中毒患者和7个对照组匹配的年龄和性别。我们首次通过LC-MS鉴定了人肾脏中的7-酮基胆固醇（7-keto）和7-羟基胆固醇（7 f -OH和7 g -OH）。在对照组的肺和肾中检测到的7 f -OOH和7 g -OOH与百草枯组一样低。与此相反，我们发现百草枯组肺中的7-酮和7 g -OH以及肾脏中的7-酮均显著高于对照组。这是首次报道百草枯中毒后氧固醇在肺和肾中的蓄积。这似乎反映了百草枯中毒病理学中更大的氧化应激。
The aim of this study is to evaluate oxidative stress in man after paraquat ingestion by analyzing 7 f - and 7 g -hydroperoxycholest-5-en-3 g -ol (7 f - and 7 g -OOH) as well as oxysterols, cholesterol oxidation products, as indices of lipid peroxidation. Lung, kidney, and liver were collected at autopsy from seven patients with paraquat poisoning and seven controls matched for age and sex. We identified for the first time 7-ketocholesterol (7-keto) and 7-hydroxycholesterol (7 f -OH and 7 g -OH) in human kidney by LC-MS. Next, we quantified 7 f -OOH and 7 g -OOH by HPLC with postcolumn chemiluminescence as well as oxysterols by HPLC-UV. Both 7 f -OOH and 7 g -OOH detected in lung and kidney from the controls were as low as the paraquat group. In contrast, we found both 7-keto and 7 g -OH in lung and 7-keto in kidney from the paraquat group were significantly higher than from the controls. This is the first report on accumulated oxysterols in lung and kidney from human paraquat poisoning. It seems to reflect greater oxidative stress in the pathology of paraquat intoxication.