Transcriptional mapping of plasmid pKK3535. Quantitation of the effect of guanosine tetraphosphate on binding to the rrnB promoters and a lambda promoter with sequence homologies in the CII binding region.
Transcriptional mapping of plasmid pKK3535. Quantitation of the effect of guanosine tetraphosphate on binding to the rrnB promoters and a lambda promoter with sequence homologies in the CII binding region.
复制标题
质粒 pKK3535 的转录图谱。
DOI：
10.1016/0022-2836(81)90041-3
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发表时间：
1981
影响因子：
5.6
通讯作者：
Chamberlin,MJ
中科院分区：
文献类型：
--
作者：
Kingston,RE;Gutell,RR;Taylor,AR;Chamberlin,MJ
我们已经在一个质粒上定位了三个启动子，该质粒含有大肠杆菌的rnB操纵子和λ的attP ′区（Brosiuset al.，1980年b）。这些启动子之一是以前未定位的启动子，称为λ PL 57，位于λ的attP ′臂上，转录从该启动子通过attcore序列进行。该启动子的序列分析揭示了与λ PI和λPE的假定CII结合序列的惊人同源性（Abrahamet al.，1980; Hoesset等人，1980），表明该启动子可能参与溶原性的建立。我们已经定位的另外两个启动子是thernB操纵子的启动子。我们用电子显微镜定量E.在存在和不存在调节核苷酸鸟苷四磷酸的情况下，将coliRNA聚合酶与这些启动子结合。这些研究表明，鸟苷四磷酸特异性地降低RNA聚合酶与每个tornB启动子结合的能力，而不影响与λ PL 57启动子的结合。
We have mapped three promoters on a plasmid that contains therrnB operon ofEscherichia coliand theattP′ region of λ (Brosiuset al., 1980b). One of these promoters is a previously unmapped promoter, termed λPL57, that is located on theattP′ arm of λ, and from which transcription proceeds through theattcore sequence. Sequence analysis of this promoter reveals striking homologies to the presumed CII binding sequences of λPIand λPE(Abrahamet al., 1980; Hoesset al., 1980), suggesting that this promoter may be involved in establishment of lysogeny. The other two promoters we have mapped are those of therrnB operon. We have used electron microscopy to quantitate the binding ofE. coliRNA polymerase to these promoters in the presence and absence of the regulatory nucleotide guanosine tetraphosphate. These studies show that guanosine tetraphosphate specifically decreases the ability of RNA polymerase to bind to each of the tworrnB promoters, while not affecting the binding to the λPL57promoter.