Identification of QTLs for seed quality traits in rapeseed (Brassica napus L.) using recombinant inbred lines (RILs)
Identification of QTLs for seed quality traits in rapeseed (Brassica napus L.) using recombinant inbred lines (RILs)
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DOI：
10.1007/s10681-016-1675-5
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发表时间：
2016-07-01
期刊：
影响因子：
1.9
通讯作者：
Lu, Yun-Hai
中科院分区：
文献类型：
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作者：
Huang, Xian-Qun;Huang, Tuan;Lu, Yun-Hai
油菜（Brassica napus L.）是中国最重要的食用油料作物，也是全球仅次于大豆的第二大食用油料作物。它为人类提供食用植物油，为饲料工业提供蛋白质，最近作为生物柴油的来源获得了越来越多的兴趣。高蛋白质、高油酸、低芥酸、低硫代葡萄糖苷含量的种子品质性状的改良仍是甘蓝型油菜最重要的育种目标。定位种子品质性状的数量性状位点（qtl）将丰富我们对这些性状的认识，提高传统油菜籽标记辅助选择育种的效率和精度。以高含油量系827R和低含油量系Darmor_Sin杂交的94个F7-8重组自交系为材料，构建了包含181个标记（143个序列相关扩增多态性（srap）和38个简单序列重复（SSRs））和19个连锁群（覆盖1307.99 cM）的遗传连锁图谱。在4个染色体区域（LG1、LG10、LG11和LG12）上共鉴定出9个品质性状qtl (LOD > 2.5)，其中油分性状4个（qOC-1、2、3、4），蛋白性状2个（qPC-1、2），油酸性状2个（qOAC-1、2），硫苷性状1个（qGLC-1）。4个含油量QTL的表型方差分别为7.70%、14.58%、16.00%和23.11%；蛋白含量qtl分别为13.16%和14.81%；两个油酸含量qtl分别为12.52%和25.48%；硫代葡萄糖苷含量QTL为47.71%。油分含量、蛋白质含量、油酸含量和硫代葡萄糖苷含量的总体表型变异分别为61.39%、27.97%、38.00%和47.71%。在鉴定的两个种子蛋白含量QTL中，一个（qPC-1）与LG1上的含油量QTL （qOC-1）紧密相连，另一个（qPC-2）与LG11上的籽油含量QTL （qOC-2）共定位。两对qtl对两个性状均有影响，加性效应相反，证实油菜籽含油量与蛋白质含量呈负相关。本研究结果将为今后深入研究甘蓝型油菜种子品质性状的分子基础和在甘蓝型油菜育种中应用MAS具有一定的参考价值。
Rapeseed (Brassica napus L.) is the most important edible oilseed crop in China and the second most important globally after soybean. It provides edible vegetable oil for human consumption and proteins for the feed industry, and has recently gained increasing interest as a source of biodiesel. The improvement of seed quality traits for high protein, oil and oleic acid, and low erucic acid and glucosinolate content remains the most important breeding goal in B. napus. Mapping of quantitative trait loci (QTLs) underlying seed quality traits will allow us to enrich knowledge on these traits and improve the efficiency and precision of conventional rapeseed breeding via marker-assisted selection (MAS). In this study, a genetic linkage map comprising 181 markers (143 sequence-related amplified polymorphisms (SRAPs) and 38 simple-sequence repeats (SSRs)) and 19 linkage groups covering 1307.99 cM was constructed with 94 F7-8 recombinant inbred lines (RILs) derived from a cross between a high oil content line '827R' and a low oil content line 'Darmor_Sin'. A total of nine QTLs were identified (LOD > 2.5) on four chromosomal regions (LG1, LG10, LG11 and LG12) for seed quality traits: four for oil content (qOC-1, 2, 3, 4), two for protein content (qPC-1, 2), two for oleic acid content (qOAC-1, 2), and one for glucosinolate content (qGLC-1). The phenotypic variance explained by individual QTL was estimated as 7.70, 14.58, 16.00, and 23.11 % respectively for the four oil content QTLs; 13.16 and 14.81 % for the two protein content QTLs; 12.52 and 25.48 % for the two oleic acid content QTLs; and 47.71 % for the glucosinolate content QTL. The overall phenotypic variation explained by the identified QTLs was 61.39 % for oil content, 27.97 % for protein content, 38.00 % for oleic acid content and 47.71 % for glucosinolate content. Of the two QTLs identified for seed protein content, one (qPC-1) was tightly linked to a QTL for oil content (qOC-1) on LG1 and another one (qPC-2) was co-localized with a second QTL for seed oil content (qOC-2) on LG11. The two pairs of QTLs affected both traits with opposite additive effects confirming the negative correlation between oil and protein content in rapeseed. The present results will be of value for ongoing efforts to dissect the molecular basis of seed quality traits and practicing MAS in B. napus breeding programs.