Multiplexing protein and gene level measurements on a single Luminex platform
Multiplexing protein and gene level measurements on a single Luminex platform
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DOI：
10.1016/j.ymeth.2019.01.018
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发表时间：
2019-04-01
期刊：
影响因子：
4.8
通讯作者：
Sebata, Thao H.
中科院分区：
文献类型：
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作者：
Cook, Damon B.;McLucas, Brian C.;Sebata, Thao H.
背景：从单个样品中同时测量多种分泌蛋白和相应基因表达水平的能力对于综合分析是有价值的。传统免疫分析和基因表达分析的瓶颈包括大样本消耗、耗时的实验程序和复杂的数据分析。方法和结果：在这里，我们展示了在Luminex平台上运行的单个样品中测量信使RNA （mRNA）表达和蛋白质的两种高通量测定方法。用脂多糖（LPS）处理人外周血单个核细胞（hPBMCs），并在24和72 h收获。用ProcartaPlex Human Immune Monitoring 65-plex Panel检测样品在QuantiGene Human 80-plex Panel上的蛋白质和相应的mRNA靶标。结论：多重ProcartaPlex和QuantiGene Plex检测提供了对蛋白水平和基因表达网络的广泛调查。
Background: The ability to simultaneously measure multiple secreted proteins and the corresponding gene expression levels from a single sample is valuable for comprehensive analysis. Bottlenecks to traditional im-munoassays and gene expression assays include large sample consumption, time consuming experimental procedures, and complex data analysis.Method and results: Here, we demonstrate two high-throughput assays measuring both messenger RNA (mRNA) expression and proteins in a single sample run on a Luminex platform. Human peripheral blood mononuclear cells (hPBMCs) were treated with lipopolysaccharide (LPS) and harvested at 24 and 72 h. Samples were assayed with the ProcartaPlex Human Immune Monitoring 65-plex Panel for protein and corresponding mRNA targets on a QuantiGene Human 80-plex Panel.Conclusion: Multiplexing ProcartaPlex and QuantiGene Plex assays provide a broad survey of protein levels and gene expression networks.