A recombinant rabies virus expressing Echinococcus granulosus EG95 induces protective immunity in mice
A recombinant rabies virus expressing Echinococcus granulosus EG95 induces protective immunity in mice
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表达细粒棘球绦虫EG95的重组狂犬病病毒诱导小鼠保护性免疫
DOI：
10.1111/tbed.14292
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发表时间：
2021
影响因子：
4.3
通讯作者：
Zheng Xuexing
中科院分区：
文献类型：
--
作者：
Xu Tong;Liu Lele;Shi Chenjuan;Liu Wenkai;Wang Min;Tian Li;Zheng Ye;Wang Hongmei;Zheng Wenwen;He Hongbin;Xia Xianzhu;Zheng Xuexing
由细粒棘球蚴（Echinococcus granulosus，E）引起的囊状棘球蚴病（Cystic Echinocococcus，CE）是一种世界性分布的人兽共患传染病，对公共卫生和社会经济造成严重影响。本研究构建了表达E.颗粒状病毒（LBNSE-EG 95）作为二价候选疫苗，用于绵羊和牛预防CE和狂犬病，这是CE流行地区的另一种严重健康威胁。结果表明，EG 95在不改变亲本LBNSE载体致病性的前提下成功表达。进一步的研究表明，LBNSE-EG 95免疫小鼠可激活树突状细胞（DCs）和B细胞，并诱导Th 1-/Th 2-介导的细胞免疫应答，从而产生大量RABV中和抗体和高水平的EG 95特异性抗体，对CE的保护率超过90%。此外，单剂量的LBNSE-EG 95在小鼠中赋予针对致死性RABV攻击的完全保护。总的来说，这些结果表明，重组LBNSE-EG 95有可能被开发为一种有效的双价疫苗，用于CE和狂犬病流行地区的绵羊和牛。
Cystic echinococcosis (CE), caused byEchinococcus granulosus(E, is a zoonosis with a worldwide distribution, resulting in heavy impact to public health and social economics. In this study, we generated a recombinant rabies virus (RABV) expressing EG95 protein ofE. granulosus(LBNSE‐EG95) as a bivalent candidate vaccine for use in sheep and cattle against CE and rabies, which is another severe health threat in CE‐endemic areas. It was found that EG95 was successfully expressed without altering the pathogenicity of parent LBNSE vector. Further study showed that LBNSE‐EG95 immunization in mice elicited activation of dendric cells (DCs) and B cells and induced Th1‐/Th2‐mediated cellular immune responses, leading to robust production of RABV neutralizing antibodies and high level of EG95‐sepecific antibodies with more than 90% protection against CE. In addition, single dose of LBNSE‐EG95 conferred full protection against lethal RABV challenge in mice. Collectively, these results suggest that the recombinant LBNSE‐EG95 has the potential to be developed as an efficient bivalent vaccine for sheep and cattle use in endemic areas of CE and rabies.