Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
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DOI：
10.1038/ncomms10098
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发表时间：
2015-12-07
影响因子：
16.6
通讯作者：
Ten Hagen KG
中科院分区：
文献类型：
--
作者：
Tran DT;Masedunskas A;Weigert R;Ten Hagen KG
The actin cytoskeleton plays crucial roles in many cellular processes, including regulated secretion. However, the mechanisms controlling F-actin dynamics in this process are largely unknown. Through 3D time-lapse imaging in a secreting organ, we show that F-actin is actively disassembled along the apical plasma membrane at the site of secretory vesicle fusion and re-assembled directionally on vesicle membranes. Moreover, we show that fusion pore formation and PIP2 redistribution precedes actin and myosin recruitment to secretory vesicle membranes. Finally, we show essential roles for the branched actin nucleators Arp2/3- and WASp in the process of secretory cargo expulsion and integration of vesicular membranes with the apical plasma membrane. Our results highlight previously unknown roles for branched actin in exocytosis and provide a genetically tractable system to image the temporal and spatial dynamics of polarized secretion in vivo. The cytoskeleton plays a crucial role in secretion. Here Tran et al. demonstrate that cortical actin is rearranged at the site of vesicle fusion and recruited to fused secretory granules in Drosophila salivary glands, and show that branched actin nucleators are required for cargo expulsion.
