Human Adipose Tissue Lipoprotein Lipase: Comparison of Assay Methods and Expressions of Activity 1
Human Adipose Tissue Lipoprotein Lipase: Comparison of Assay Methods and Expressions of Activity 1
复制标题
人脂肪组织脂蛋白脂肪酶：测定方法和活性表达的比较 1
DOI：
10.3181/00379727-148-38526
复制
发表时间：
1975
影响因子：
--
通讯作者：
J. Brunzell
中科院分区：
文献类型：
--
作者：
O. Pykälistö;Phillip H. Smith;J. Brunzell
正常人脂肪组织中肝素可释放脂蛋白脂酶与氢氧化铵丙酮-乙醚匀浆脂蛋白脂酶呈正相关。释放的肝素作为脂蛋白脂肪酶活性约为丙酮粉末中酶活性的两倍，即使在与肝素孵育后，40-70%的原始活性仍保留在组织中。这可能表明酶的激活与肝素从组织中释放有关。每单位体重和每个脂肪细胞的脂蛋白脂肪酶活性受到肥胖的不同影响：在肥胖受试者中，每单位体重的脂蛋白脂肪酶成比例地低于每个脂肪细胞的活性。每个脂肪细胞的活性的表达似乎避免了肥胖的影响，因此增加了脂肪细胞的大小，对所获得的值。
Summary There was a positive correlation in normal man between heparin releasable lip-oprotein lipase and lipoprotein lipase of ammonium hydroxide homogenate of acetone-ether powder in adipose tissue. Heparin releasable as lipoprotein lipase activity was about twice as high as the enzymatic activity in acetone powder, even though 40-70% of the original activity remained in the tissue after incubation with heparin. This might indicate that activation of the enzyme is associated with its release by heparin from tissue. The lipoprotein lipase activity per unit weight and per fat cell were affected differently by obesity: In obese subjects lipoprotein lipase per unit weight was proportionally lower than the activity per fat cell. The expression of activity per fat cell appears to avoid the effect of obesity, and hence increased fat cell size, on values obtained.