Characterization of the immunologic responses to human in vivo acquired enamel pellicle as a novel means to investigate its composition
Characterization of the immunologic responses to human in vivo acquired enamel pellicle as a novel means to investigate its composition
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DOI：
10.1034/j.1399-302x.2003.00065.x
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发表时间：
2003-06-01
影响因子：
--
通讯作者：
Oppenheim, FG
中科院分区：
文献类型：
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作者：
Li, J;Helmerhorst, EJ;Oppenheim, FG
人类获得的釉质膜是由选择性吸附在牙齿表面的分子形成的。本工作描述了使用单抗(MAb)技术作为一种新的方法来鉴定膜中存在的微量成分。获得的单抗与他汀类、组蛋白、粘液糖蛋白1(MG1)、白蛋白、淀粉酶和人免疫球蛋白(IGs)具有反应活性，表明这些单抗是膜成分，免疫印迹进一步证实了这一点。没有获得针对富含脯氨酸蛋白(PRPS)、溶菌酶、粘液糖蛋白2(MG2)、碳酸氢酶、乳铁蛋白或过氧化物酶的单抗，这表明这些成分不存在、含量低或抗原性低。对所获得的部分抗MG1、抗他汀类和抗组素单抗的结合表位进行了进一步的鉴定，并讨论了其生物学意义。这一结果为利用人膜免疫和mAbs产物鉴定未知成分提供了可能。
Human acquired enamel pellicle is formed by molecules selectively adsorbed onto tooth surfaces. The present work describes the use of monoclonal antibody (mAb) technology as a novel approach to identify micro amounts of components present in pellicle. MAbs were obtained with reactivities against statherin, histatin, mucous glycoprotein 1 (MG1), albumin, amylase and human immunoglobulins (Igs), indicating that these are pellicle components, which was further confirmed by immunoblotting. No mAbs against proline-rich proteins (PRPs), lysozyme, mucous glycoprotein 2 (MG2), carbonic anhydrase, lactoferrin or peroxidase were obtained, suggesting that these components are absent, present in low amounts, or exhibit low antigenicity. Further characterization of the binding epitopes of some of the obtained anti-MG1, anti-statherin and anti-histatin mAbs were carried out and the biological relevance is discussed. The results open up the possibility that immunization with human pellicle and mAbs production can be employed to identify hitherto unknown constituents of pellicle.