ELECTROTONIC COUPLING BETWEEN PYRAMIDAL CELLS - A DIRECT DEMONSTRATION IN RAT HIPPOCAMPAL SLICES
ELECTROTONIC COUPLING BETWEEN PYRAMIDAL CELLS - A DIRECT DEMONSTRATION IN RAT HIPPOCAMPAL SLICES
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DOI：
10.1126/science.6266013
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发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
DUDEK, FE
中科院分区：
文献类型：
--
作者：
MACVICAR, BA;DUDEK, FE
大鼠海马切片中成对神经元的细胞内记录直接证明了CA3锥体细胞之间的电紧张耦合。当2个神经元被同时刺穿（如通过随后的辣根过氧化物酶双重染色所验证的）时，注入1个细胞的电流脉冲引起其他细胞的电压变化。这些相互作用是双向的。快速前电位，历史上被认为代表树突中的尖峰活动，是由其他电紧张耦合的锥体细胞中的动作电位引起的。这些数据直接证明了哺乳动物大脑神经元之间的电紧张耦合，并表明一些快前电位是耦合电位。锥体细胞之间的耦合可以介导癫痫发作期间正常节律活动和爆发性放电的同步化。
Intracellular recordings from pairs of neurons in slices of rat hippocampus directly demonstrated electrotonic coupling between CA3 pyramidal cells. When 2 neurons were impaled simultaneously (as verified by subsequent double staining with horseradish peroxidase), current pulses injected into 1 cell caused voltage changes in other cells. These interactions were bidirectional. Fast prepotentials, historically thought to represent spike activity in dendrites, resulted from action potentials in other electrotonically coupled pyramidal cells. The data directly demonstrate electrotonic coupling between neurons in the mammalian brain and indicate that some fast prepotentials are coupling potentials. Coupling between pyramidal cells could mediate synchronization of normal rhythmic activity and of burst discharges during seizures.