Array-based profiling of ragweed and mugwort pollen allergens
Array-based profiling of ragweed and mugwort pollen allergens
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DOI：
10.1111/j.1398-9995.2008.01780.x
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发表时间：
2008-11-01
期刊：
影响因子：
12.4
通讯作者：
Hawranek, T.
中科院分区：
文献类型：
--
作者：
Gadermaier, G.;Wopfner, N.;Hawranek, T.
背景：豚草(Ambrosia Artemisiifolia)和艾草(Artemisia Uggaris)花粉是引起夏末和秋季过敏反应的主要原因。豚草和艾草花粉过敏的鉴别诊断是过敏专科医生经常遇到的问题，因为这两种植物具有相同的抗原结构和重叠的花季。目的：通过基因芯片和酶联免疫吸附试验(EL ISA)评估杂草过敏患者对大量纯化的过敏原的致敏模式。方法：从天然来源中纯化8种豚草和6种艾属花粉变应原，或在大肠杆菌中以重组蛋白的形式表达。过敏原被发现在蛋白质微阵列载玻片上或包被在酶标板上。结果：所有豚草过敏个体均对AmbA-1致敏，其中30%对豚草主要变应原单敏。艾花粉过敏患者中，对ART V1和ART V3的认知度最高。在两组患者中都观察到了广泛的交叉反应，主要涉及泛过敏原Profilin和非特异性脂转移蛋白。用变应原芯片和酶联免疫吸附试验两种方法获得相似的IgE图谱。结论：基于分子的诊断为豚草和艾草花粉过敏的鉴别诊断和为特定免疫治疗选择合适的过敏原提供了必要的信息。
Background: Ragweed (Ambrosia artemisiifolia) and mugwort (Artemisia vulgaris) pollen is the main cause of allergic reactions in late summer and autumn. The differential diagnosis between ragweed and mugwort pollen allergy is a frequent problem encountered by allergologists in areas where both plants are present due to shared antigenic structures and overlapping flowering seasons.Objective: To evaluate the sensitization pattern of weed allergic patients towards a large panel of purified allergens in the microarray format and by enzyme-linked immunosorbent assay (ELISA).Methods: Eight ragweed and six mugwort pollen allergens were purified from natural source or expressed as recombinant proteins in Escherichia coli. Allergens were spotted on protein microarray slides or coated onto ELISA plates. Sera from 19 ragweed and/or mugwort allergic individuals were used to determine the reactivity towards single molecules in both assays.Results: All ragweed allergic individuals were sensitized to Amb a 1, among them 30% were monosensitized to the major ragweed allergen. Art v 1 and Art v 3 were recognized by 89% of mugwort pollen-allergic patients. Extensive cross-reactivity was observed for both patient groups mainly involving the pan-allergens profilin and nonspecific lipid transfer proteins. Comparable IgE profiles were obtained with both allergen microarray and ELISA methods. Conclusions: Molecule-based diagnosis provides essential information for the differential diagnosis between ragweed and mugwort pollen allergy and for the selection of the appropriate allergen source for specific immunotherapy.