4-1BB expression on MAIT cells is associated with enhanced IFN-γ production and depends on IL-2
4-1BB expression on MAIT cells is associated with enhanced IFN-γ production and depends on IL-2
复制标题
MAIT 细胞上的 4-1BB 表达与 IFN-γ 产生增强相关，并且依赖于 IL-2
DOI：
10.1016/j.cellimm.2018.03.013
复制
发表时间：
2018-06-01
影响因子：
4.3
通讯作者：
Cheng, Xiaoxing
中科院分区：
文献类型：
--
作者：
Jiang, Jing;Cao, Zhihong;Cheng, Xiaoxing
MAIT细胞在人类抗结核分枝杆菌感染免疫中的作用仍在很大程度上未被探索。在这项研究中，我们研究了4-1BB对MAIT细胞的功能作用。我们发现4-1BB在结核性胸腔积液的MAIT细胞上在Mtb抗原刺激后高度上调，其表达水平与IFN-γ和IL-17的产生相关。MAIT细胞上响应Mtb抗原的4-1BB表达部分依赖于IL-2，并且与共同的γ链受体相关。通过转录组测序，我们鉴定了4-1BB（-）和4-1BB（+）MAIT细胞之间的许多差异表达基因。差异表达基因的GO富集和KEGG途径分析鉴定了富集的途径，包括T细胞受体和NF-κ B信号传导途径。结论：4-1BB有可能被用作生物标志物，以确定MAIT细胞与增强IFN-γ和IL-17的反应，可能与结核病感染控制。
The role of MAIT cells in immunity against Mycobacterium tuberculosis infection in humans is still largely unexplored. In this study, we investigated the functional role of 4-1BB on MAIT cells. We found that 4-1BB was highly up-regulated on MAIT cells from tuberculous pleural effusions following Mtb antigen stimulation and its level of expression correlated with IFN-gamma and IL-17 production. 4-1BB expression on MAIT cells in response to Mtb antigens was partially dependent on IL-2 and was associated with common gamma chain receptor. By transcriptome sequencing, we identified numerous differentially expressed genes between 4-1BB(-) and 4-1BB(+) MAIT cells. GO enrichment and KEGG pathway analysis of differentially expressed genes identified enriched pathways that included T-cell receptor and NF-kappa B signaling pathways. It is concluded that 4-1BB has the potential to be used as a biomarker to identify MAIT cells with enhanced IFN-gamma and IL-17 responses that might be associated with tuberculosis infection control.