Protective effects of standardized pomegranate (Punica granatum L.) polyphenolic extract in ultraviolet-irradiated human skin fibroblasts
Protective effects of standardized pomegranate (Punica granatum L.) polyphenolic extract in ultraviolet-irradiated human skin fibroblasts
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DOI：
10.1021/jf8005307
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发表时间：
2008-09-24
影响因子：
6.1
通讯作者：
Mertens-Talcott, Susanne U.
中科院分区：
文献类型：
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作者：
Pacheco-Palencia, Lisbeth A.;Noratto, Giuliana;Mertens-Talcott, Susanne U.
暴露于紫外线（UV）辐射与几种急性和慢性疾病有关，包括晒伤、水肿、增生、免疫抑制、光老化和皮肤癌。天然存在的植物化学物质在预防这种与紫外线有关的疾病中的作用引起了越来越多的兴趣。石榴（Punica granatum L.）是多酚类物质的丰富来源，在许多体内和体外研究中已被证明具有抗炎、抗氧化和抗癌活性。本研究探讨了石榴果提取物对UVA和uvb诱导的SKU-1064人皮肤成纤维细胞损伤的潜在保护作用。石榴提取物（PE），在5至60 mg/L的范围内，可以有效地保护人类皮肤成纤维细胞免受紫外线照射后的细胞死亡，可能与促炎转录因子NF-kappa B的激活减少，促凋亡caspase-3的下调以及与DNA修复相关的G0/G1期的增加有关。需要更高的多酚浓度（500-10000 mg/L）才能显著降低紫外线诱导的活性氧水平，提高细胞内抗氧化能力（从1.9 μ M Trolox当量/mL增加到8.6 μ M Trolox当量/mL）。本研究的结果证明了PE对UVA和uvb诱导的细胞损伤的保护作用，以及石榴多酚类物质在局部应用中的潜在应用。
Exposure to ultraviolet (UV) radiation has been associated with several acute and chronic conditions, including sunburn, edema, hyperplasia, immunosuppression, photoaging, and skin cancer. The role of naturally occurring phytochemicals in the prevention of such UV-related conditions has captured increased interest. Pomegranate (Punica granatum L.) is a rich source of polyphenolics, which have been shown to exert anti-inflammatory, antioxidant, and anticarcinogenic activity in numerous in vivo and in vitro studies. This work investigated potential protective effects of a pomegranate fruit extract standardized to punicalagins against UVA- and UVB-induced damage in SKU-1064 human skin fibroblast cells. Pomegranate extract (PE), in a range from 5 to 60 mg/L, was effective at protecting human skin fibroblasts from cell death following UV exposure, likely related to a reduced activation of the pro-inflammatory transcription factor NF-kappa B, a downregulation of proapoptotic caspase-3, and an increased G0/G1 phase, associated with DNA repair. Higher polyphenolic concentrations (500-10000 mg/L) were needed to achieve a significant reduction in UV-induced reactive oxygen species levels and increased intracellular antioxidant capacity (from 1.9 to 8.6 mu M Trolox equivalents/mL). Results from this study demonstrate the protective effects of PE against UVA- and UVB-induced cell damage and the potential use of pomegranate polyphenolics in topical applications.