Enhanced growth of seed viruses for H5N1 influenza vaccines
Enhanced growth of seed viruses for H5N1 influenza vaccines
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DOI：
10.1016/j.virol.2007.07.002
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发表时间：
2007-09-15
期刊：
影响因子：
3.7
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Horimoto, Taisuke;Murakami, Shin;Kawaoka, Yoshihiro
用于生产灭活H5N1流感疫苗的种子病毒是重组病毒，含有修改的无毒型血凝素(HA)和完整的神经氨酸酶(NA)基因，这两种基因都来自H5N1分离株，以及所有剩余的基因来自在鸡蛋中生长良好的PR8株。然而，一些重组菌在鸡蛋中生长得不太理想，这给及时、经济高效的疫苗生产带来了障碍。在这里，我们证明了我们的PR8毒株比世卫组织推荐的用于种子病毒NIBRG-14的PR8毒株对卵子生长的支持更好。此外，加入另一种NA蛋白进一步促进了鸡蛋中的病毒生长。这些发现表明，我们的H5N1候选疫苗将在新的大流行开始时增加H5N1疫苗的可用剂量。(C)2007 Elsevier Inc.保留所有权利。
Seed viruses used to produce inactivated H5N1 influenza vaccines are recombinant viruses with modified avirulent-type hernagglutinin (HA) and intact neuraminidase (NA) genes, both derived from an H5N1 isolate, and all remaining genes from the PR8 strain, which grows well in eggs. However, some reassortants grow suboptimally in eggs, imposing obstacles to timely, cost-efficient vaccine production. Here, we demonstrate that our PR8 strain supports better in ovo growth than the PR8 strain used for the WHO-recommended seed virus, NIBRG-14. Moreover, inclusion of an alternative NA protein further enhanced viral growth in eggs. These findings suggest that our H5N1 vaccine candidates would increase the availability of H5N I vaccine doses at the onset of a new pandemic. (c) 2007 Elsevier Inc. All rights reserved.