Protists are an integral part of the Arabidopsis thaliana microbiome
Protists are an integral part of the Arabidopsis thaliana microbiome
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DOI：
10.1111/1462-2920.13941
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发表时间：
2018
影响因子：
5.1
通讯作者：
M. Sapp;S. Ploch;A. Fiore-Donno;M. Bonkowski;L. Rose
中科院分区：
文献类型：
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作者：
M. Sapp;S. Ploch;A. Fiore-Donno;M. Bonkowski;L. Rose
虽然原生生物占据了广泛的栖息地，并通过疾病抑制，竞争或生长刺激等方式与植物相互作用，但它们对“植物生物群系”的贡献还没有得到很好的描述。为了更全面地了解植物全生生物，我们研究了与生长在两种不同土壤中的模式植物拟南芥（Arabidopsis thaliana）相关的尾虫和卵菌类群。在表面消毒之前和之后，对土壤、根、叶和木制牙签进行了分析。用18S rRNA基因元巴编码鉴定尾虫，用内转录间隔区1鉴定卵菌。随后的分析显示，强大的空间结构的原生生物群落之间的车厢，虽然卵菌出现更专业比尾藻。在卵菌方面，只有霜霉目和球孢囊菌属的成员被鉴定为A.拟南芥微生物组这也适用于属于Glissomonadida和Cercomonadida的尾虫分类群。我们确定了根际土壤因素的强烈影响，但不是叶际。明显的差异，尾虫发现，最好是在木材或新鲜的植物材料意味着特定的生态位适应。我们的研究结果突出了微型真核生物的重要性，植物holobiont。
Although protists occupy a vast range of habitats and are known to interact with plants among other things via disease suppression, competition or growth stimulation, their contributions to the 'phytobiome' are not well described. To contribute to a more comprehensive picture of the plant holobiont, we examined cercozoan and oomycete taxa living in association with the model plant Arabidopsis thaliana grown in two different soils. Soil, roots, leaves and wooden toothpicks were analysed before and after surface sterilization. Cercozoa were identified using 18S rRNA gene metabarcoding, whereas the Internal Transcribed Spacer 1 was used to determine oomycetes. Subsequent analyses revealed strong spatial structuring of protist communities between compartments, although oomycetes appeared more specialized than Cercozoa. With regards to oomycetes, only members of the Peronosporales and taxa belonging to the genus Globisporangium were identified as shared members of the A. thaliana microbiome. This also applied to cercozoan taxa belonging to the Glissomonadida and Cercomonadida. We identified a strong influence by edaphic factors on the rhizosphere, but not for the phyllosphere. Distinct differences of Cercozoa found preferably in wood or fresh plant material imply specific niche adaptations. Our results highlight the importance of micro-eukaryotes for the plant holobiont.