A CRITICAL-EVALUATION OF METHODS FOR ESTIMATING THE NUMERICAL DENSITY OF SYNAPSES
A CRITICAL-EVALUATION OF METHODS FOR ESTIMATING THE NUMERICAL DENSITY OF SYNAPSES
复制标题
DOI：
10.1016/0165-0270(86)90113-5
复制
发表时间：
1986-10-01
影响因子：
3
通讯作者：
BIERMAN, EPB
中科院分区：
文献类型：
--
作者：
DEGROOT, DMG;BIERMAN, EPB
描述了估计颗粒数值密度 (Nv) 的几种方法。讨论了不同方法在估计突触密度方面的有用性和局限性。这些方法是：（1）离散展开技术，（2）串行切片技术和（3）解剖器技术。从结果可以得出结论，不建议使用展开技术来估计突触的数量和大小，因为有关形状、截断和过度投影的各种假设都是危险的。与串行切片技术的结果相比，使用解剖器技术获得的 Nv 值始终较低。讨论了通过两种无偏技术获得的这种差异。关于这一点的主要结论是，只要获得切片厚度的可靠估计并使用适当的采样程序，这两种技术都可以用于估计突触密度。
Several methods for estimating the numerical density (Nv) of particles are described. The usefulness and the limitations of different methods with respect to the estimation of synaptic densities are discussed. These methods are: (1) the discrete unfolding technique, (2) the serial section technique and (3) the disector technique.From the results it is concluded that it is not advisable to use an unfolding technique to estimate the number and size of synapses since all sorts of assumptions regarding the shape, truncation and overprojection are hazardous.Consistently lower values forNvwere obtained with the disector technique compared with the results of the serial section technique. This difference, obtained with two unbiased techniques, is discussed. The main conclusion with respect to this point is that both techniques can be used to estimate synaptic densities, provided a reliable estimate of the section thickness is obtained and an appropriate sampling procedure is used.