Spatiotemporal quantification of subcellular ATP levels in a single HeLa cell during changes in morphology.
Spatiotemporal quantification of subcellular ATP levels in a single HeLa cell during changes in morphology.
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DOI：
10.1038/srep16874
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发表时间：
2015-11-17
影响因子：
4.6
通讯作者：
Oka K
中科院分区：
文献类型：
--
作者：
Suzuki R;Hotta K;Oka K
细胞形状变化与 ATP 消耗之间的定量关系是细胞生物学中尚未解决的问题。在这项研究中，同步成像和图像处理分析使我们首次能够在生理条件下观察和量化这些关系。我们关注细胞的两个边缘区域：富含微管的“片层”和富含肌动蛋白的“外围结构”。同时成像和相关分析表明，微管动力学导致层状形状变化，同时 ATP 水平增加。此外，图像处理和时空量化能够可视化突出长度和 ATP 水平之间关系的时间变化，并且表明它们正在相互影响。此外，微管动力学的抑制减少了外周结构的运动性和片层中 ATP 水平的波动范围。这项工作清楚地表明细胞运动和形态受到微管和肌动蛋白动力学之间与 ATP 相关的协作功能的调节。
The quantitative relationship between change in cell shape and ATP consumption is an unsolved problem in cell biology. In this study, a simultaneous imaging and image processing analysis allowed us to observe and quantify these relationships under physiological conditions, for the first time. We focused on two marginal regions of cells: the microtubule-rich ‘lamella’ and the actin-rich ‘peripheral structure’. Simultaneous imaging and correlation analysis revealed that microtubule dynamics cause lamellar shape change accompanying an increase in ATP level. Also, image processing and spatiotemporal quantification enabled to visualize a chronological change of the relationships between the protrusion length and ATP levels, and it suggested they are influencing each other. Furthermore, inhibition of microtubule dynamics diminished motility in the peripheral structure and the range of fluctuation of ATP level in the lamella. This work clearly demonstrates that cellular motility and morphology are regulated by ATP-related cooperative function between microtubule and actin dynamics.