Duplicate isochorismate synthase genes of Bacillus subtilis: regulation and involvement in the biosyntheses of menaquinone and 2,3-dihydroxybenzoate.
Duplicate isochorismate synthase genes of Bacillus subtilis: regulation and involvement in the biosyntheses of menaquinone and 2,3-dihydroxybenzoate.
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枯草芽孢杆菌的重复异分支酸合酶基因：调节和参与甲基萘醌和 2,3-二羟基苯甲酸酯的生物合成。
DOI：
10.1128/jb.178.3.854-861.1996
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发表时间：
1996
影响因子：
3.2
通讯作者：
Taber,HW
中科院分区：
文献类型：
--
作者：
Rowland,BM;Taber,HW
枯草芽孢杆菌具有重复的异分支酸合酶基因menF和dhbC。异分支酸合酶参与呼吸链组分甲萘醌（MK）和铁载体2，3-二羟基苯甲酸酯（DHB）的生物合成。构建了几种menF和dhbC缺失突变体以鉴定每个基因产物对MK和DHB生物合成的贡献。menF缺失突变体能够产生野生型水平的MK和DHB，表明dhbC基因产物能够补偿MenF的缺乏。然而，dhbC缺失突变体产生野生型水平的MK，但DHB缺乏，表明MenF无法弥补DhbC的缺乏。menF dhbC双缺失突变体是MK和DHB缺陷的。转录分析表明，dhbC的表达，而不是menF，是由铁浓度调节。构建dhbA ′：：lacZ融合菌株以检测突变对dhb启动子区内铁盒序列的影响。这些突变消除了dhB基因的铁调节转录，表明在B中存在Fur样阻遏蛋白。枯草杆菌。
Bacillus subtilis has duplicate isochorismate synthase genes, menF and dhbC. Isochorismate synthase is involved in the biosynthesis of both the respiratory chain component menaquinone (MK) and the siderophore 2,3-dihydroxybenzoate (DHB). Several menF and dhbC deletion mutants were constructed to identify the contribution made by each gene product to MK and DHB biosynthesis. menF deletion mutants were able to produce wild-type levels of MK and DHB, suggesting that the dhbC gene product is able to compensate for the lack of MenF. However, a dhbC deletion mutant produced wild-type levels of MK but was DHB deficient, indicating that MenF is unable to compensate for the lack of DhbC. A menF dhbC double-deletion mutant was both MK and DHB deficient. Transcription analysis showed that expression of dhbC, but not of menF, is regulated by iron concentration. A dhbA'::lacZ fusion strain was constructed to examine the effects of mutations to the iron box sequence within the dhb promoter region. These mutations abolished the iron-regulated transcription of the dhb genes, suggesting that a Fur-like repressor protein exists in B. subtilis.