In vivo transcriptomic profile after a Mediterranean diet in high-cardiovascular risk patients: a randomized controlled trial
In vivo transcriptomic profile after a Mediterranean diet in high-cardiovascular risk patients: a randomized controlled trial
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DOI：
10.3945/ajcn.113.060582
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发表时间：
2013-09-01
影响因子：
7.1
通讯作者：
Fito, Montserrat
中科院分区：
文献类型：
--
作者：
Castaner, Olga;Corella, Dolores;Fito, Montserrat
背景：营养素可通过营养基因组调控发挥健康作用。关于持续的传统地中海饮食(TMD)模式对整个转录反应的体内影响的数据很少。目的：我们探讨了与TMD相关的总体营养经济学效应。设计：我们在Prevencion Con Dieta地中海(PREDIMED)研究的一个子样本(n=34)中重点关注与心血管疾病(CVD)相关的生物学途径，这是一项大型、平行分组、多中心、随机对照试验，旨在评估TMD对心血管风险高的个人的心血管疾病一级预防的影响。参与者被随机分配到等量的低脂饮食对照组或TMD干预组[传统地中海饮食加初榨橄榄油(TMD+VOO)或传统地中海饮食加坚果(TMD+坚果)]。用全转录组微阵列分析评估全基因组外周血单核细胞三个月的变化。结果：对TMD+VOO后的241个应答基因(上调139个，下调102个)、TMD+坚果后的312个应答基因(上调165个、下调147个)、低脂饮食后的145个选定应答基因(100个上调、45个下调)进行功能注释分析。在18条心血管典型通路分析中，有12条通路有差异表达，其中43%的通路受两种TMD的调节；最常见的通路与动脉粥样硬化和高血压有关。经过同步测试调整，TMD+VOO饮食调节9条通路，TMD+坚果饮食调节4条通路。结论：TMD，特别是在补充初榨橄榄油的情况下，发挥健康益处的机制之一是通过改变与心血管风险相关的基因的转录反应。这项试验在伦敦的现行对照试验登记处登记为ISRCTN35739639。
Background: Nutrients can exert healthy effects through nutrigenomic modulation. Data are scarce concerning the in vivo effect of a sustained traditional Mediterranean diet (TMD) pattern on the whole transcriptomic response. Objective: We explored the overall nutrigenomic effect associated with a TMD. Design: We focused on biological pathways related to cardiovascular disease (CVD) in a subsample (n = 34) of the Prevencion Con Dieta Mediterranea (PREDIMED) study, which was a large, parallel-group, multicenter, randomized controlled trial that aimed to assess the effects of TMD on the primary prevention of CVD in individuals with high cardiovascular risk. Participants were randomly assigned to a low-fat diet control group or TMD intervention groups [traditional Mediterranean diet supplemented with virgin olive oil (TMD+VOO) or traditional Mediterranean diet supplemented with nuts (TMD+Nuts)] in equal proportions. Three-month changes in whole genome peripheral blood mononuclear cells were assessed by using whole transcriptome microarray analyses. Results: A functional annotation analysis was performed on 241 selected responder genes after the TMD+VOO (139 upregulated and 102 downregulated genes), 312 selected responder genes after the TMD+Nuts (165 upregulated and 147 downregulated genes), and 145 selected responder genes after the low-fat (100 upregulated and 45 downregulated genes) diets. Of 18 cardiovascular canonical pathway analyses, 12 pathways were differentially expressed, and 43% of pathways were modulated by both TMDs; the most prevalent pathways were related to atherosclerosis and hypertension. After simultaneous testing adjustment, 9 pathways were modulated by the TMD+VOO diet, and 4 pathways were modulated by the TMD+Nuts diet. Conclusion: One of the mechanisms by which TMD, particularly if supplemented with virgin olive oil, can exert health benefits is through changes in the transcriptomic response of genes related to cardiovascular risk. This trial was registered at the London-based Current Controlled Trials register as ISRCTN35739639.