Photophysiological responses of intertidal benthic microalgal communities to in situ light environments: Methodological considerations
Photophysiological responses of intertidal benthic microalgal communities to in situ light environments: Methodological considerations
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潮间带底栖微藻群落对原位光环境的光生理反应：方法学考虑
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
R. Zingmark
中科院分区：
文献类型：
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作者：
J. Pinckney;R. Zingmark
光合作用对辐照度（P-I）曲线通常以每单位生物量的产量表示产量。然而，对于底栖微藻群落，沉积物透光带的深度以及暴露于光的生物量随入射辐照度的变化而变化。采用传统的深度积分法、百分比P、、、和固定深度间隔法研究了不同光环境下潮间带底栖微藻群落的光合响应。P-1曲线参数（a，I，，I，，）根据所用方法而变化。用深度综合生物量比方法计算，无遮荫滩涂微藻的（Y）（0.261 kO.016）高于有遮荫的高米草微藻（0.16 1 + O.O14），表明滩涂微藻比高米草微藻更能适应遮荫。然而，如果构建曲线，在固定深度（表面）的沉积物中获得的测量，高米草微藻表现出显着较高的（Y（0.260-1-0.020）比mudllat微藻（0.175 kO.006）。采用后一种方法，高米草表面微藻比泥滩表面微藻出现更多的阴影驯化。我们的结论是，固定深度的间隔方法提供了一个更真实的代表性的底栖微藻群落的生理反应。
Photosynthesis vs. irradiance (P-l) curves usually express production in terms of production per unil biomass. However, for benthic microalgal communities, the depth of the sediment photic zone as well as the amount of biomass exposed to light varies as a function of incident irradiance. We examined the photosynthetic responses of intertidal benthic microalgal communities from different light environments with traditional depth-integrated methods as well as the percent P,,, and fixed-depth interval methods. P-lcurve parameters (a, I,, I,,,) change depending on the method used. With the depth-integrated biomassspecific method, microalgae in unshaded mudflats have a higher (Y (0.261 kO.016) than microalgae in shaded tall Spartina habitats (0.16 1 +_O.O 14), suggesting that mudflat microalgae are more shade-acclimated than tall Spartina microalgae. However, if curves are constructed,with measurements obtained at a lixed depth (surface) in the sediment, tall Spartina microalgae exhibit a significantly higher (Y (0.260-1-0.020) than mudllat microalgac (0.175 kO.006). With the latter method, tall Spartina surface microalgae appear more shade-acclimated than mudflat surface microalgae. We conclude that the fixed-depth interval method provides a more realistic representation of the photophysiological responses of benthic microalgal communities.