Removal of mercury from its aqueous solution using charcoal-immobilized papain (CIP)
Removal of mercury from its aqueous solution using charcoal-immobilized papain (CIP)
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DOI：
10.1016/j.jhazmat.2009.07.085
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发表时间：
2009-12-30
影响因子：
13.6
通讯作者：
Basu, Srabanti
中科院分区：
文献类型：
--
作者：
Dutta, Susmita;Bhattacharyya, Aparupa;Basu, Srabanti
在目前的工作中，汞已根除其水溶液中使用木瓜蛋白酶，固定在活性炭上的物理吸附法。木瓜蛋白酶在活性炭上吸附的操作参数如pH、活性炭的量、溶液中木瓜蛋白酶的初始浓度已经以合适的方式改变，用于标准化操作条件，以基于其比酶活性获得最佳固定化木瓜蛋白酶样品。当木瓜蛋白酶初始浓度为40.0g/L、活性炭用量为0.5g、pH为7时，固定化木瓜蛋白酶的比酶活最高。该样品已被指定为炭固定化木瓜蛋白酶（CIP），并用于汞去除的进一步研究。木瓜蛋白酶在活性炭上的吸附平衡数据符合Langmuir吸附等温式。已完成CIP的理化表征。在实验室规模的间歇接触器中，研究了CIP法从氯化汞模拟溶液中去除汞的效果。操作参数即，溶液中汞的初始浓度、CIP的量和pH值以规定的方式变化。当初始金属浓度为20.0 mg/L，CIP投加量为0.03 g时，在pH为7时，最大去除率可达99.4%。最后，在不同的pH值下进行了汞的解吸研究，以评估汞的回收过程。这样得到的结果是令人满意的。(C)2009爱思唯尔有限公司版权所有。
In the present work mercury has been eradicated from its aqueous solution using papain, immobilized on activated charcoal by physical adsorption method. Operating parameters for adsorption of papain on activated charcoal like pH, amount of activated charcoal, initial concentration of papain in solution have been varied in a suitable manner for standardization of operating conditions for obtaining the best immobilized papain sample based on their specific enzymatic activity. The immobilized papain sample obtained at initial papain concentration 40.0 g/L, activated charcoal amount 0.5 g and pH 7 shows the best specific enzymatic activity. This sample has been designated as charcoal-immobilized papain (CIP) and used for further studies of mercury removal. Adsorption equilibrium data fit most satisfactorily with the Langmuir isotherm model for adsorption of papain on activated charcoal. Physicochemical characterization of CIP has been done. The removal of mercury from its simulated solution of mercuric chloride using CIP has been studied in a lab-scale batch contactor. The operating parameters viz., the initial concentration of mercury in solution, amount of CIP and pH have been varied in a prescribed manner. Maximum removal achieved in the batch study was about 99.4% at pH 7, when initial metal concentration and weight of CIP were 20.0 mg/L and 0.03 g respectively. Finally, the study of desorption of mercury has been performed at different pH values for assessment of recovery process of mercury. The results thus obtained have been found to be satisfactory. (C) 2009 Elsevier B.V. All rights reserved.