Increase in muscle endurance in mice by dietary Yamabushitake mushroom ( Hericium erinaceus ) possibly via activation of PPAR δ
Increase in muscle endurance in mice by dietary Yamabushitake mushroom ( Hericium erinaceus ) possibly via activation of PPAR δ
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食用山布什菌（Hericium erinaceus）可能通过激活 PPAR δ 来增加小鼠的肌肉耐力
DOI：
10.1111/asj.13199
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发表时间：
2019
影响因子：
2
通讯作者：
Mizunoya Wataru
中科院分区：
文献类型：
--
作者：
Komiya Yusuke;Nakamura Toshiya;Ishii Momoko;Shimizu Kuniyoshi;Hiraki Eri;Kawabata Fuminori;Nakamura Mako;Tatsumi Ryuichi;Ikeuchi Yoshihide;Mizunoya Wataru
骨骼肌纤维主要分为两种类型：1型（慢缩）和2型（快缩）纤维。肉的品质和纤维类型的组成被认为是密切相关。先前的研究表明，组成型活性过氧化物酶体增殖物激活受体（过氧化物酶体增殖物激活受体）δ（一种存在于骨骼肌中的核受体）的过度表达增加了小鼠的1型纤维。在本研究中，我们发现猴头菌（Hericium erinaceus）的正己烷提取物在体外具有激活过氧化物酶体增殖物激活受体δ（PPARδ）的活性。给8周龄C57 BL/6 J小鼠喂食补充有5%（w/w）冻干山武蘑菇的饮食24小时。治疗期结束后，切除趾长伸肌（EDL）。补充山香菇的饲料在体内上调小鼠骨骼肌中的PPARδ靶基因Pdk 4和Ucp 3。此外，给小鼠喂食补充Yamabushitake的饮食8周，导致肌肉耐力显著增加。这些结果表明，山香菇中含有PPARδ激动性配体，膳食摄入山香菇可激活小鼠骨骼肌中的PPARδ。出乎意料的是，我们观察到在喂食对照和添加Yamabushitake的饮食的小鼠之间，肌纤维类型的组成没有显著变化。
Skeletal muscle fiber is largely classified into two types: type 1 (slow‐twitch) and type 2 (fast‐twitch) fibers. Meat quality and composition of fiber types are thought to be closely related. Previous research showed that overexpression of constitutively active peroxisome proliferator‐activated receptor (PPAR)δ, a nuclear receptor present in skeletal muscle, increased type 1 fibers in mice. In this study, we found that hexane extracts of Yamabushitake mushroom (Hericium erinaceus) showed PPARδ agonistic activityin vitro. Eight‐week‐old C57BL/6J mice were fed a diet supplemented with 5% (w/w) freeze‐dried Yamabushitake mushroom for 24 hr. After the treatment period, the extensor digitorum longus (EDL) muscles were excised. The Yamabushitake‐supplemented diet up‐regulated the PPARδ target genesPdk4andUcp3in mouse skeletal musclesin vivo. Furthermore, feeding the Yamabushitake‐supplemented diet to mice for 8 weeks resulted in a significant increase in muscle endurance. These results indicate that Yamabushitake mushroom contains PPARδ agonistic ligands and that dietary intake of Yamabushitake mushroom could activate PPARδ in skeletal muscle of mice. Unexpectedly, we observed no significant alterations in composition of muscle fiber types between the mice fed control and Yamabushitake‐supplemented diets.