Species identification, antifungal susceptibility, and clinical feature association of Aspergillus section Nigri isolates from the lower respiratory tract
Species identification, antifungal susceptibility, and clinical feature association of Aspergillus section Nigri isolates from the lower respiratory tract
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下呼吸道黑曲霉分离株的物种鉴定、抗真菌药敏性和临床特征关联
作者：
Takeda Keita;Suzuki Junko;Watanabe Akira;Matsuki Mei;Higa Katsuyuki;Inoue Eri;Akashi Shunsuke;Shimada Masahiro;Kawashima Masahiro;Ohshima Nobuharu;Fukami Takeshi;Masuda Kimihiko;Yamane Akira;Tamura Atsuhisa;Nagai Hideaki;Matsui Hirotoshi;Tohma Shigeto;Kam
黑曲霉组的种通常通过分子遗传学方法鉴定，而在临床实践中，它们被归类阿萨。它们的形态特征决定了它们的起源。本研究旨在探讨呼吸道分离的黑曲霉菌的种类是否因临床诊断而不同。从2012年2月至2017年1月，在国立医院组织（NHO）东京国立医院收集了从43例患者的下呼吸道分离的44株黑曲霉菌分离株。根据β-微管蛋白基因分析进行种属鉴定。药敏试验按临床和实验室标准协会（CLSI）M38第3版进行，并对临床特点进行回顾性分析。welwitschiae的分离频率最高，其次为A. tubingensis）。A.与只有一个A相比，tubingensisisolates表现出对唑类的低敏感性。孤立的大约四分之三的来自WhomA. welwitschiae被分离的患者中有一半以上被诊断为定植，而来自A.分离出结核杆菌病患者，确诊为慢性肺曲霉病（CPA）。结论：CPA合并黑曲霉感染的患者，应加强药物选择。在这些患者中，发现经常耐唑的塔宾氏菌是最普遍的。
Species ofAspergillussectionNigriare generally identified by molecular genetics approaches, whereas in clinical practice, they are classified asA. nigerby their morphological characteristics. This study aimed to investigate whether the species ofAspergillussectionNigriisolated from the respiratory tract vary depending on clinical diagnosis. Forty-fourAspergillussectionNigriisolates isolated from the lower respiratory tracts of 43 patients were collected from February 2012 to January 2017 at the National Hospital Organization (NHO) Tokyo National Hospital. Species identification was carried out based on β-tubulin gene analysis. Drug susceptibility tests were performed according to the Clinical and Laboratory Standards Institute (CLSI) M38 3rd edition, and the clinical characteristics were retrospectively reviewed.A. welwitschiaewas isolated most frequently, followed byA. tubingensis. More than half of theA. tubingensisisolates exhibited low susceptibility to azoles in contrast to only oneA. welwitschiaeisolate. Approximately three quarters of the patients from whomA. welwitschiaewas isolated were diagnosed with colonization, whereas more than half the patients from whomA. tubingensiswas isolated were diagnosed with chronic pulmonary aspergillosis (CPA). More attention needs to be given to the drug choice for patients with CPA withAspergillussectionNigriinfection becauseA. tubingensis, which was found to be frequently azole-resistant, was the most prevalent in these patients.