Test characteristics of two rapid antigen detection tests (SD FK50 and SD FK60) for the diagnosis of malaria in returned travellers.
Test characteristics of two rapid antigen detection tests (SD FK50 and SD FK60) for the diagnosis of malaria in returned travellers.
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DOI：
10.1186/1475-2875-8-90
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发表时间：
2009-05-05
期刊：
影响因子：
3
通讯作者：
Jacobs J
中科院分区：
文献类型：
--
作者：
Van der Palen M;Gillet P;Bottieau E;Cnops L;Van Esbroeck M;Jacobs J
在旅行诊所环境中评估了两种疟疾快速诊断测试：SD FK50疟疾银恶性疟原虫测试（两波段测试）和SD FK60疟疾银恶性疟原虫/Pan测试（三波段测试）。使用了一组来自返回旅行者的储存全血样本（FK50和FK60分别为n = 452和n = 614）。结果不一致的参照方法为PCR镜检。两种方法对恶性疟原虫的总检测灵敏度分别为93.5%，在寄生虫密度大于100和1000 /μl时，检测灵敏度分别为97.6%和100%。FK60检测对间日疟原虫、卵形疟原虫和疟疾疟原虫的总敏感性分别为87.5%、76.3%和45.2%，而在寄生虫密度大于500/μl时，间日疟原虫和卵形疟原虫的总敏感性分别为92.6%和90.5%。所有物种的特异性均在95%以上，两种检测方法经PCR校正后，均可见疟疾疟原虫（n = 3）、间日疟原虫和卵形疟原虫（各1个样本）的富组氨酸蛋白-2系。株系强度具有可重复性，且与寄生虫密度相关。FK60试验为恶性疟原虫密度≤1000 /μl提供了线索。FK50和FK60对恶性疟原虫的诊断效果较好，而FK60对间日疟原虫和卵形疟原虫的诊断效果较好。FK60作为半定量估计寄生虫密度的潜在用途有待进一步探索。
Two malaria rapid diagnostic tests were evaluated in a travel clinic setting: the SD FK50 Malaria Ag Plasmodium falciparum test (a two-band test) and the SD FK60 Malaria Ag P. falciparum/Pan test (a three-band test). A panel of stored whole blood samples (n = 452 and n = 614 for FK50 and FK60, respectively) from returned travellers was used. The reference method was microscopy with PCR in case of discordant results. For both tests, overall sensitivity for the detection of P. falciparum was 93.5%, reaching 97.6% and 100% at parasite densities above 100 and 1,000/μl respectively. Overall sensitivities for Plasmodium vivax, Plasmodium ovale and Plasmodium malariae for the FK60 test were 87.5%, 76.3% and 45.2%, but they reached 92.6% and 90.5% for P. vivax and P. ovale at parasite densities above 500/μl. Specificities were above 95% for all species and both tests when corrected by PCR, with visible histidine-rich protein-2 lines for P. malariae (n = 3) and P. vivax and P. ovale (1 sample each). Line intensities were reproducible and correlated to parasite densities. The FK60 tests provided clues to estimate parasite densities for P. falciparum below or above 1,000/μl. Both the FK50 and FK60 performed well for the diagnosis of P. falciparum in the present setting, and the FK60 for the diagnosis of P. vivax and P. ovale at parasite densities > 500/μl. The potential use of the FK60 as a semi-quantitative estimation of parasite density needs to be further explored.
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影响因子：
14.2
作者：
H채nscheid, T
通讯作者：
H채nscheid, T
影响因子：
--
作者：
Gatti, S.;Gramegna, M.;Scaglia, M.
通讯作者：
Scaglia, M.
影响因子：
9.4
作者：
Playford, EG;Walker, J
通讯作者：
Walker, J
DOI：
10.1016/s0035-9203(98)91013-6
发表时间：
1998-05-01
影响因子：
2.2
作者：
Van den Ende, J;Vervoort, T;Lynen, L
通讯作者：
Lynen, L
DOI：
10.4269/ajtmh.1999.60.109
发表时间：
1999-01-01
影响因子：
3.3
作者：
Piper, R;Lebras, J;Makler, M
通讯作者：
Makler, M