The effect of parasympathetic postganglionic denervation on parotid salivary protein secretion in anaesthetized sheep
The effect of parasympathetic postganglionic denervation on parotid salivary protein secretion in anaesthetized sheep
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DOI：
10.1016/s1566-0702(02)00148-0
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发表时间：
2002-09-30
影响因子：
2.7
通讯作者：
Titchen, DA
中科院分区：
文献类型：
--
作者：
Edwards, AV;Titchen, DA
对麻醉后21 ~ 28天的羊进行了腮腺单侧副交感神经去神经支配的观察。副交感神经去支配减少了同侧腺体的体积，同时增加了对侧腺体的体积，以至于腺体的总体积大于正常神经支配腺体的羊。正常对照动物去神经支配腺的自发分泌量(8.8 +/- 1.1 mul min（-1) g腺（-1））明显低于有神经支配腺(26.0 +/- 2.7 mul min(-1) g腺（-1）；P < 0.0 1)，蛋白质含量较高。当考虑到体重差异时，正常神经支配的和慢性去神经支配的腺体对交感刺激的流量和钠钾的输出是相似的，而副交感神经去神经支配后蛋白质的输出显着增强(神经支配-31.4 +/- 7.3马克克腺（-1），去神经支配-83.4 +/- 26.6马克克腺（-1）；P < 0.05)。动脉内灌注乙酰胆碱(130 pmol min（-1) kg(-1)）引起腮腺唾液流动，经事先副交感神经去神经处理后腮腺唾液蛋白含量显著增加。动脉内灌注血管活性肠肽(VIP; 2.5 pmol min（-1) kg(-1)）可使对侧受神经支配而非无神经支配腺体的腮腺唾液流量增加（P < 0.05）。它还引起蛋白质输出的少量增加，这是通过事先去神经支配而显著增强的。VIP对腮腺对乙酰胆碱的反应无协同作用。结果表明，羊腮腺的副交感神经支配对腮腺具有重要的营养作用。肾上腺素能受体和胆碱能受体的相互作用对刺激蛋白质分泌有重要贡献，而事先去神经支配增强了蛋白质对乙酰胆碱和VIP的反应。(C) 2002 Elsevier Science B.V.版权所有
Effects of unilateral parasympathetic denervation of ovine parotid glands were examined in anaesthetized sheep 21-28 days after nerve section. Parasympathetic denervation reduced the mass of the ipsilateral gland while increasing that of the contralateral gland to the extent that total gland mass was greater than in sheep with normally innervated glands. The spontaneous secretion (8.8 +/- 1.1 mul min(-1) g gland(-1)) was significantly less from denervated than from innervated glands of normal control animals (26.0 +/- 2.7 mul min(-1) g gland(-1); P < 0.0 1) and contained more protein. Rates of flow, and the outputs of sodium and potassium, in response to sympathetic stimulation, were similar from normally innervated and chronically denervated glands, when allowance was made for the discrepancy in weights, whereas the output of protein was significantly enhanced following parasympathetic denervation (innervated -31.4 +/- 7.3 mug g gland(-1), denervated-83.4 +/- 26.6 mug g gland(-1); P < 0.05). Intra-arterial infusions of acetylcholine (130 pmol min(-1) kg(-1)) elicited a flow of parotid saliva, the protein content of which was significantly enhanced by prior parasympathetic denervation. Intra-arterial infusions of vasoactive intestinal peptide (VIP; 2.5 pmol min(-1) kg(-1)) produced a small but statistically significant (P < 0.05) increase in the flow of parotid saliva from the contralateral, innervated but not from denervated glands. It also caused a small increase in protein output, which was significantly enhanced by prior denervation. VIP had no synergistic effect on the parotid responses to acetylcholine. The results show that the parasympathetic innervation to the parotid gland of the sheep exerts important trophic effects on the gland. Interaction of adronergic and cholinergic receptors makes an important contribution to stimulation of the secretion of protein and prior denervation potentiates the protein responses to both acetylcholine and VIP. (C) 2002 Elsevier Science B.V. All rights reserved.