Identification and expression of carbonic anhydrase 2, myosin regulatory light chain 2 and selenium-binding protein 1 in zebrafish Danio rerio: Implication for age-related biomarkers
Identification and expression of carbonic anhydrase 2, myosin regulatory light chain 2 and selenium-binding protein 1 in zebrafish Danio rerio: Implication for age-related biomarkers
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斑马鱼斑马鱼碳酸酐酶 2、肌球蛋白调节轻链 2 和硒结合蛋白 1 的鉴定和表达：对年龄相关生物标志物的影响
DOI：
10.1016/j.gep.2018.04.007
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发表时间：
2018-09-01
影响因子：
1.2
通讯作者：
Li, Hongyan
中科院分区：
文献类型：
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作者：
Feng, Shuoqi;Wang, Su;Li, Hongyan
蛋白质组学研究已经确定了绝经后妇女肌肉中碳酸氢酶2、肌球蛋白调节轻链2和硒结合蛋白1的合成随年龄的变化。然而，关于这些蛋白在早期发育和生命周期中的表达和作用的信息很少。在这项研究中，我们发现斑马鱼CA2、Myl2a、Myl2b和Selenbp1在一级和三级结构以及基因组组织和同线图谱上与它们的哺乳动物同源。它们在斑马鱼的胚胎和幼体中表现出不同的时空表达模式。此外，CA2、MYL2a、MYL2b和SELENBP1在各组织中的转录水平随着年龄的增长而明显改变，即有的表达上调，有的表达不变甚至降低，提示CA2、MYL2a、MYL2b和SELENBP1可作为衰老的生物标志物。我们的研究也为进一步阐明这些基因在早期发育和衰老过程中的功能奠定了基础。
Proteomic study has determined age-related changes in synthesis of carbonic anhydrase 2, myosin regulatory light chain 2 and selenium-binding protein 1 in muscle of post-menopausal women. However, little information is available regarding the expression and role of these proteins in early development and life span. In this study we showed that zebrafish ca2, myl2a, myl2b and selenbp1 were highly identical to their mammalian counterparts in primary and tertiary structures as well as genomic organization and syntenic map. They displayed distinct spatiotemporal expression patterns in embryos and larvae of zebrafish. Moreover, their transcription levels in the respective tissues were obviously remodeled in an age-dependent fashion, i.e. some mRNA levels were increased, while others remained unchanged or even decreased, suggesting that CA2, MYL2a, MYL2b and SELENBP1 can be used as aging biomarkers. Our study also lays a foundation for further illumination of the functions of these genes in early development and aging processes.