Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis.
Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis.
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DOI：
10.1007/978-1-4939-6448-2_31
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Basile JR
中科院分区：
文献类型：
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作者：
Zhou H;Yang YH;Basile JR
信号素和丛状蛋白是一个富含半胱氨酸的细胞表面和分泌蛋白家族，最初被发现控制神经生长和免疫反应，但最近被发现参与了广泛的发育和病理过程，这些过程受到细胞黏附和迁移的影响。沿着这些思路，我们的团队和其他人发现Semaphorin 4D(SEMA4D)通过促进表达其受体Plexin-B1的内皮细胞的趋化作用在血管生成中发挥重要作用。事实上，一些肿瘤会与其他促血管生成蛋白一起产生SEMA4D，目的是促进血管生长成为正在发展的肿瘤。在此，我们介绍了体外迁移和小管生成试验以及体内定向血管生成试验(DIVAA)在测量细胞来源和可溶性SEMA4D血管生成潜力方面的应用。
The semaphorins and plexins comprise a family of cysteine-rich cell surface and secreted proteins originally shown to control nerve growth and the immune response, but that have recently been implicated in a wide variety of developmental and pathological processes that are influenced by cell adhesion and migration. Along those lines, our group and others have found that Semaphorin 4D (SEMA4D) plays an important role in angiogenesis by promoting chemotaxis of endothelial cells, which express its receptor, Plexin-B1. Indeed, some neoplasms produce SEMA4D along with other pro-angiogenic proteins for the purpose of enhancing blood vessel growth into a developing neoplasm. Here we describe the application of in vitro migration and tubulogenesis assays and the Directed In Vivo Angiogenesis Assay (DIVAA) in the measurement of the angiogenic potential of cell-derived and soluble SEMA4D.