Development and validation of a method for the analysis of tetracyclines in chicken-muscle by liquid chromatography-electrospray-mass spectrometry in tandem (LC-ESI-MS/MS)
Development and validation of a method for the analysis of tetracyclines in chicken-muscle by liquid chromatography-electrospray-mass spectrometry in tandem (LC-ESI-MS/MS)
复制标题
DOI：
10.1590/s0100-40422011000100009
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Gomes, Luiz Nelson F
中科院分区：
文献类型：
--
作者：
Uekane, Thais Matsue;Aquino Neto, Francisco Radler;Gomes, Luiz Nelson F
由于欧洲是巴西产品的主要市场之一，因此根据欧盟决议2002/657/EC开发并验证了用于测定鸡肉中四环素类（TC）的LC-ESI-MS/MS方法。线性相关系数r > 0.9979，定量限为7.0- 35.0ng/g，平均回收率为89.38 ~ 106.27%，日内、日间精密度良好。方法的检出限为93.00-106.46 ng/g，检测能力为95.84-114.38 ng/g。该方法适用于鸡肉中四环素类抗生素残留的监测。
A LC-ESI-MS/MS method was developed and validated according to the European Union decision 2002/657/EC, for the determination of tetracyclines (TCs) in chicken-muscle since Europe is one of the main markets for Brazilian products. Linearity of r > 0.9979, limits of quantification in the range of 7.0-35.0 ng/g, average recoveries of 89.38 - 106.27%, within-day and between-day precision were adequate for all TCs. The decision limit and the detection capability were 93.00-106.46 ng/g and 95.84-114.38 ng/g, respectively. This method is suitable for application in surveillance programmes of residues of TCs in chicken-muscle samples.