In vivo imaging of graft-versus-host-disease in mice
In vivo imaging of graft-versus-host-disease in mice
复制标题
DOI：
10.1182/blood-2003-08-2827
复制
发表时间：
2004-05-01
期刊：
影响因子：
20.3
通讯作者：
Blazar, BR
中科院分区：
文献类型：
--
作者：
Panoskaltsis-Mortari, A;Price, A;Blazar, BR
我们开发了一种小鼠系统，通过该系统，在骨髓移植（BMT）诱导的移植物抗宿主病（GVHD）环境中，使用增强型绿色荧光蛋白（eGFP）转基因（Tg）细胞全身输注后，通过该系统跟踪细胞的迁移和归巢。简单应用配备彩色电荷耦合器件（CCD）相机的荧光立体显微镜。在细胞输注后的不同时间点拍摄的麻醉小鼠的全身图像显示同种异体细胞早期迁移到外周淋巴器官，随后浸润GVHD靶器官。通过剃毛小鼠的皮肤可以看到eGFP Tg细胞的定位，并且内部器官容易辨别。在同种异体或同基因eGFP Tg细胞输注后，解剖代表性小鼠以更好地可视化更深的内部器官和组织。不同细胞群的输注揭示了不同的归巢模式，这种方法也提供了一种简单的方法来确定移植细胞在各种器官中扩增的关键时间点。这种简单的应用程序的荧光立体显微镜将是有价值的GVHD和移植物抗肿瘤的研究，其中可视化的细胞迁移，扩张，细胞间的相互作用将是更多的信息时，通过这样的活体方法进行分析。(C)2004年，美国血液学会。
We have developed a mouse system by which to track the migration and homing of cells in a setting of bone marrow transplantation (BMT)-induced graft-versus-host disease (GVHD) after systemic infusion using enhanced green fluorescence protein (eGFP) transgenic (Tg) cells and a simple application of a fluorescence stereomicroscope outfitted with a color charge-coupled device (CCD) camera. Whole body images of anesthetized mice taken at various time points after cell infusion revealed the early migration of allogeneic cells to peripheral lymphoid organs, with later infiltration of GVHD target organs. Localization of eGFP Tg cells could be seen through the skin of shaved mice, and internal organs were easily discernible. After allogeneic or syngeneic eGFP Tg cell infusion, representative mice were dissected to better visualize deeper internal organs and tissues. Infusion of different cell populations revealed distinct homing patterns, and this method also provided a simple way to identify the critical time points for expansion of the transplanted cells in various organs. This simple application of the fluorescence stereomicroscope will be valuable for GVHD and graft-versus-tumor studies in which visualization of cellular migration, expansion, and cell-cell interactions will be more informative when analyzed by such an intravital method. (C) 2004 by The American Society of Hematology.