Determination of some estrogens by flow injection analysis with acidic potassium permanganate-formaldehyde chemiluminescence detection
Determination of some estrogens by flow injection analysis with acidic potassium permanganate-formaldehyde chemiluminescence detection
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DOI：
10.1016/j.aca.2005.01.005
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发表时间：
2005-04-29
影响因子：
6.2
通讯作者：
Xie, ZH
中科院分区：
文献类型：
--
作者：
Liao, SL;Wu, XP;Xie, ZH
建立了一种快速、简便、灵敏地测定双烯雌酚、己烯雌酚和己烯雌酚三种雌激素的新型流动注射化学发光法。它是基于所研究的雌激素与甲醛（HCHO）和高锰酸钾（KMnO4）在硫酸（H2SO4）介质中的CL反应。在最佳条件下，双烯雌酚的线性范围为8.00 × 10(-9) ~ 8.00 × 10(-6) g ml(-1)，己烯雌酚的线性范围为5.00 × 10(-9) ~ 4.75 × 10(-6) g ml(-1)，己烯雌酚的线性范围为2.00 × 10(-8) ~ 5.00 × 10(-6) g ml（-1）。双烯雌酚的检出限（S/N = 3）分别为2.61 × 10(-9) g ml（-1）、1.08 × 10(-9) g ml（-1）和6.28 × 10(-9) g ml（-1）。该方法已成功地应用于片剂和自来水中雌激素的测定。并对可能的反应机理进行了探讨。(c) 2005 Elsevier B.V.版权所有
A novel flow injection chemiluminescence (CL) method for the fast, simple and sensitive determination of three estrogens, namely dienestrol, diethylstilbestrol and hexestrol, has been investigated. It is based upon the CL reaction of the studied estrogens with formaldehyde (HCHO) and potassium permanganate (KMnO4) in sulfuric acid (H2SO4) medium. Under the optimum conditions, the linear range for the determination of estrogens were 8.00 x 10(-9) to 8.00 x 10(-6) g ml(-1) for dienestrol, 5.00 x 10(-9) to 4.75 x 10(-6) g ml(-1) for diethylstilbestrol, and 2.00 x 10(-8) to 5.00 x 10(-6) g ml(-1) for hexestrol. The detection limits (S/N = 3) were 2.61 x 10(-9) g ml(-1) for dienestrol, 1.08 x 10(-9) g ml(-1) for diethylstilbestrol and 6.28 x 10(-9) g ml(-1) for hexestrol. The proposed method has been successfully applied to the determination of the studied estrogens in tablets and tap water. Furthermore, the possible reaction mechanism was also discussed in this paper. (c) 2005 Elsevier B.V. All rights reserved.